Recombinant ESAT-6 (6kD Early Secretory Antigen of T cells, Mycobacterium Tuberculosis, MTB)
Catalog No : USB-209170
473.58€
0.00€
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| Product name | Recombinant ESAT-6 (6kD Early Secretory Antigen of T cells, Mycobacterium Tuberculosis, MTB) | ||
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| Catalog No | USB-209170 | ||
| Supplier’s Catalog No | 209170 | ||
| Supplier | US Biologicals | ||
| Source antigen | Recombinant, E. coli | ||
| Reactivity | |||
| Cross reactivity | |||
| Applications | |||
| Molecular weight | |||
| Storage | -20°C | ||
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| Other names | |||
| Grade | Highly Purified | ||
| Purity | Highly Purified (~98%) | ||
| Form | Supplied as a liquid in NaPo4, pH 7.0, 0.1M sodium chloride, 0.05% sarkosyl. | ||
| Reactivity life | 12 months | ||
| Note | For reserch purpose only | ||
| Purity | Highly Purified (~98%) | ||
| Description | Tuberculosis, MTB, or TB (short for tubercle bacillus) is a common, and in many cases lethal, infectious disease caused by various strains of mycobacteria, usually Mycobacterium tuberculosis. Tuberculosis typically attacks the lungs, but can also affect other parts of the body. Individuals with HIV are at risk for infection by tuberculosis due to their impaired immune system. The only currently available vaccine as of 2012 is bacillus Calmette-Guérin (BCG with live attenuated bacteria) which, while it is effective against disseminated disease in childhood, confers inconsistent protection against contracting pulmonary TB. A number of new TB vaccines are currently in phase I and II clinical trials. MVA85A (modified vaccinia Ankara 85A) is a subunit vaccine to BCG. The closely related proteins of the antigen 85 complex are major secreted products of mycobacteria. Three closely related components, termed antigens 85A, 85B, and 85C, have been demonstrated in M. bovis BCG and M. tuberculosis. Although the antigens are genetically distinct, they are highly homologous and cross-react with polyclonal and monoclonal antibodies raised against individual components. Sequence analysis revealed 85% identity between the M. bovis BCG 85A and 85B. Many other mycobacterial antigens have been identified, such as 71, 65, 38, 23, 19, 16, 14 and 12kD proteins. The 38kD protein is an immunodominant lipoprotein antigen is specific only for the M. tuberculosis complex. It is the most extensively studied antigen. The 16kD antigen is an immunodominant antigen, frequently called 14kD, related to the family of low molecular weight heat-shock proteins. This antigen contains B-cell epitopes specific for the M. tuberculosis complex. Mycobacterium antigen ESAT-6 has been isolated from low molecular weight fractions of the shot-term-culture filtrate (ST-CF) and it can easily be detected in tuberculosis patients. This antigen includes many epitopes detectable in the serum of most patients with tuberculosis (more than 90%). Recently it was shown that ESAT-6 is very potential as diagnostic for differentiation between the mycobacterial infection and BCG vaccination. CFP-10 also known as ESAT-6-like protein esxB or secreted antigenic protein MTSA-10 or 10kD culture filtrate antigen CFP-10 is a protein that is encoded by the esxB gene. CFP-10 is a 10kD secreted antigen from Mycobacterium tuberculosis. It forms a 1:1 heterodimeric complex with ESAT-6. Both genes are expressed from the RD1 region of the bacterial genome and play a key role in the virulence of the infection. Source: Recombinant protein corresponding to ESAT-6, expressed in E. coli. Specificity Recognizes human TB serum in ELISA and Western ELISA. Human serum polyclonal antibodies yield titers of 1:1000 or greater at 100ng of immobilized protein under standard ELISA conditions. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: ELISA: 10-100ng protein Western Blot: 10-100ng protein Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | ||
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