SARS Associated Spike Mosaic S(N), Recombinant

Catalog No : USB-209193
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Product name SARS Associated Spike Mosaic S(N), Recombinant
Catalog No USB-209193
Supplier’s Catalog No 209193
Supplier US Biologicals
Source antigen Recombinant, E. coli
Reactivity
Cross reactivity
Applications
Molecular weight 38
Storage -20°C
Other names
Grade Highly Purified
Purity ~95% (10% PAGE, coomassie staining)
Form Supplied as a liquid in 25mM Tris-HCl, 0.4% sarcosyl, 0.25% Triton-100, 50% glycerol.
Reactivity life 12 months
Note For reserch purpose only
Purity ~95% (10% PAGE, coomassie staining)
Description SARS Coronavirus is an enveloped virus containing three outer structural proteins, namely the membrane (M), envelope (E), and spike (S) proteins. Spike (S)-glycoprotein of the virus interacts with a cellular receptor and mediates membrane fusion to allow viral entry into susceptible target cells. Accordingly, S-protein plays an important role in virus infection cycle and is the primary target of neutralizing antibodies. Source: Recombinant protein corresponding to SARS Associated Spike Mosaic S(N), contains the middle section of the Spike protein aa408-470, aa540-557, expressed in E. coli. Specificity: Immunoreactive with sera of SARS-infected individuals. Molecular Weight: ~38kD Applications: Suitable for use in ELISA, Western Blot and as an Antigen for detection. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.