SARS-CoV Nucleocapsid, Recombinant, aa1-422, His-Tag

Catalog No : USB-209194
473.58€
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Product name SARS-CoV Nucleocapsid, Recombinant, aa1-422, His-Tag
Catalog No USB-209194
Supplier’s Catalog No 209194
Supplier US Biologicals
Source antigen Recombinant, E. coli
Reactivity
Cross reactivity
Applications
Molecular weight 47.8
Storage -20°C
Other names
Grade Highly Purified
Purity ~95% (SDS-PAGE)
Form Supplied as a lyophilized powder in 0.05M acetate, pH 4. Reconstitute with 200ul of sterile 0.1M acetate buffer, pH 4 and let the lyophilized pellet dissolve completely.
Reactivity life 12 months
Note For reserch purpose only
Purity ~95% (SDS-PAGE)
Description SARS Coronavirus is an enveloped virus containing three outer structural proteins, namely the membrane (M), envelope (E), and spike (S) proteins. Spike (S)-glycoprotein of the virus interacts with a cellular receptor and mediates membrane fusion to allow viral entry into susceptible target cells. Accordingly, S-protein plays an important role in virus infection cycle and is the primary target of neutralizing antibodies. Source: Recombinant protein corresponding to SARS-CoV Nucleocapsid, containing 422aa residues of the SARS-CoV Nucleocapsid protein and 15 additional aa, fused to his-tag at N-terminal, expressed in E. coli. Molecular Weight: ~47.8kD AA Sequence: The aa sequence of the recombinant SARS-CoV Nucleocapsid protein is 100% homologous to aa sequence of the native SARS- CoV Nucleocapsid protein. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Reconstitute with sterile buffer. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.