Treponema pallidum, p47, Recombinant

Catalog No : USB-209261
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Product name Treponema pallidum, p47, Recombinant
Catalog No USB-209261
Supplier’s Catalog No 209261
Supplier US Biologicals
Source antigen Recombinant, E. coli
Reactivity
Cross reactivity
Applications
Molecular weight
Storage -20°C
Other names
Grade Highly Purified
Purity ~95% (10% PAGE, coomassie staining)
Form Supplied as a liquid in 70mM Tris-HCl, pH 8.0, 50mM sodium chloride, 1.5M urea, 50% glycerol.
Reactivity life 12 months
Note For reserch purpose only
Purity ~95% (10% PAGE, coomassie staining)
Description Treponema pallidum is a gram-negative spirochaete bacterium and is considered to be metabolically crippled. There are at least four known subspecies: T. pallidum pallidum, T. pallidum pertenue, T. pallidum carateum and T. pallidum endemicum. The helical structure of T. pallidum pallidum allows it to move in a corkscrew motion through viscous mediums such as mucus. Treponema pallidum sub sp. pallidum has one of the smallest bacterial genomes at 1.14 million base pairs (Mb) and has limited metabolic capabilities, reflecting its adaptation through genome reduction to the rich environment of mammalian tissue. Source: Recombinant protein corresponding to T. Pallidum p47 immunodominant regions, contains beta-galactosidase (~114kD) fused at N-terminus, expressed in E. coli. Specificity: Immunoreactive with sera of T. Pallidum infected individuals. Applications: Suitable for use in ELISA, Western Blot and as an Antigen for the detection of T. Pallidum with minimal specificity problems. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.