West Nile, Pre-M, Recombinant, His-Tag

Catalog No : USB-209305
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Product name West Nile, Pre-M, Recombinant, His-Tag
Catalog No USB-209305
Supplier’s Catalog No 209305
Supplier US Biologicals
Source antigen Recombinant, E. coli
Reactivity
Cross reactivity
Applications
Molecular weight 20
Storage -20°C
Other names
Grade Highly Purified
Purity ~95% (10% PAGE, coomassie staining).
Form Supplied as a liquid in 20mM phosphate, pH 7.5.
Reactivity life 12 months
Note For reserch purpose only
Purity ~95% (10% PAGE, coomassie staining).
Description West Nile virus (WNV) is a virus of the family Flaviviridae part of the Japanese encephalitis (JE) antigenic complex of viruses. Image reconstructions and cryoelectron microscopy reveal a 45-50nm virion covered with a relatively smooth protein surface. This structure is similar to virus; both belong to the genus flavivirus within the family Flaviviridae. WNV is a positive-sense, single strand of RNA, it is between 11,000 and 12,000 nucleotides long which encode seven non-structural proteins and three structural proteins. The RNA strand is held within a nucleocapsid formed from 12kD protein blocks; the capsid is contained within a host-derived membrane altered by two viral glycoproteins. Source: Recombinant protein corresponding to the West-Nile N-Terminal Pre-M Virus immunodominant regions, fused to 6x his-tag, expressed in E. coli. Molecular Weight: ~20kD Applications: Suitable for use in ELISA, Western Blot and as an Antigen for detection of West-Nile virus with minimal specificity problems. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.