Bacillus anthracis, Protective Antigen, Activated (PA 63), Recombinant (Anthrax)
Catalog No : USB-215909
991.98€
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| Product name | Bacillus anthracis, Protective Antigen, Activated (PA 63), Recombinant (Anthrax) | ||
|---|---|---|---|
| Catalog No | USB-215909 | ||
| Supplier’s Catalog No | 215909 | ||
| Supplier | US Biologicals | ||
| Source antigen | Recombinant | ||
| Reactivity | |||
| Cross reactivity | |||
| Applications | |||
| Molecular weight | 63 | ||
| Storage | -20°C | ||
|---|---|---|---|
| Other names | |||
| Grade | Highly Purified | ||
| Purity | ~95% (Densitometric analysis) | ||
| Form | Supplied as a lyophilized powder in 10mM bis-Tris propane, 0.1M sodium chloride, pH 9.0, 1.25% trehalose. Reconstitute with 500ul sterile ddH2O. Handle Gently. Do not vortex. | ||
| Reactivity life | 12 months | ||
| Note | For reserch purpose only | ||
| Purity | ~95% (Densitometric analysis) | ||
| Description | Bacillus anthracis is the etiological agent of anthrax. Major virulence factors produced by Bacillus anthracis are the gamma-linked, poly-D-glutamic acid capsule and an exotoxin composed of three components, protective antigen (PA), lethal factor (LF), and edema factor (EF). These proteins are not toxic separately but act in binary combinations. The complex of PA, the cell binding component, with LF, one of the enzymatic moieties, is termed “lethal toxin” and can cause death. PA and the enzymatic EF together cause skin edema. After secretion, PA is cleaved by membrane peptidases, allowing the 63kD carboxy terminal fragment to oligomerize to a heptamer. Cleavage is an essential step in exposing the binding sites for EF and LF. The assembled PA complex and associated LF or EF enters the cell through endocytosis. PA mediates the transfer of LF and EF to the cytoplasm where these enzymes recognize and proteolyze their targets. The LF component, a zinc-dependent metalloprotease, cleaves the mitogen-activated protein kinase kinase (MAPKK), impairing its function and thereby blocking the MAPK signaling pathway. EF is a calmodulin-dependent adenylate cyclase which creates in the cell excess amounts of cyclic-AMP, resulting in edema. Current research is focused on the development of an effective vaccine as well as screening for potent inhibitors of the LF enzymatic activity. Activated protective antigen (PA 63) has been prepared by trypsinization of protective antigen followed by removal of the 20kD cleavage product. PA 63 forms an oligomer and binds to both lethal factor (LF) and edema factor (EF). Source: Recombinant protein corresponding to activated Protective Antigen 63 from Bacillus anthracis. Molecular Weight: ~63kD Endotoxin: 1.16EU/mg (Kinetic chromagenic LAL assay) Recommended Dilution: For tissue culture applications, medium containing glutamine must be fresh. Ammonium ions are released when glutamine breaks down, and may prevent acidification of the endosome thereby inhibiting translocation of LF or EF into the cytosol. A stable form of glutamine must be used Storage and Stability: Lyophilized powder may be stored at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | ||
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