Binary Toxin, A Subunit, Recombinant, Clostridium Difficile, aa44-463
Catalog No : USB-215912
706.92€
0.00€
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| Product name | Binary Toxin, A Subunit, Recombinant, Clostridium Difficile, aa44-463 | ||
|---|---|---|---|
| Catalog No | USB-215912 | ||
| Supplier’s Catalog No | 215912 | ||
| Supplier | US Biologicals | ||
| Source antigen | Recombinant, E. coli | ||
| Reactivity | |||
| Cross reactivity | |||
| Applications | |||
| Molecular weight | 48.68 | ||
| Storage | -20°C | ||
|---|---|---|---|
| Other names | |||
| Grade | Highly Purified | ||
| Purity | ~95% (Densitometric analysis) | ||
| Form | Supplied as a lyophilized powder in 20mM HEPES, pH 7.4, 1.25% lactose. Reconstitute with 100ul sterile ddH2O. Handle Gently. Do not vortex. | ||
| Reactivity life | 12 months | ||
| Note | For reserch purpose only | ||
| Purity | ~95% (Densitometric analysis) | ||
| Description | A major nosocomial pathogen, Clostridium difficile causes antibiotic-associated colitis and intestinal Inflammatory disease, together called CDI. By releasing two large protein enterotoxins, Toxin A (TcdA) and Toxin B (TcdB), Clostridium difficile mediates inflammatory diarrhea and compromises intestinal epithelial cells. Either toxin when administered to cultured cells disrupts the cytoskeleton and sets off capsase-dependent apoptosis, although, TcdB is many times more potent than TcdA. Both toxins also activate intestinal epithelial and immune cells to produce cytokines and chemokines, likely contributing to the complications associated with severe CDI. Intracellular targets of TcdA and TcdB are RhoGTPases which regulate many host cell processes including establishing an epithelial barrier and the migration and signaling of immune cells. When the Rho proteins are glycosylated by Toxins A or B, signaling is disrupted leading to the intestinal damage and inflamation characteristic of CDI. Source: Recombinant protein corresponding to aa44-463 from full-length Clostridium difficile Binary toxin, A subunit, omitting the leader sequence, containing five residual aa from the affinity tag at the N-terminus, expressed in E. coli. Molecular Weight: ~48.68kD Biological Activity: Tested in an in vitro ADP-ribosylation assay. The activity was measured in 30mM sodium phosphate, pH 7.0, 7mM beta-NAD, 20mM dithiothreitol, 30mM agmantine as the ADP-ribosyl acceptor. The reaction is initiated by addition of the A subunit and incubation proceeds for 90 minutes at 30°C. The nicotinamide generated is monitored using reverse phase HPLC. Specific Activity: ~22nmoles of nicotinamide/mg of toxin/min. Endotoxin: ~3000EU/mg (Kinetic chromogenic LAL assay) Storage and Stability: Lyophilized powder may be stored at -20°C. Reconstitute with sterile buffer or ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | ||
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