Exotoxin A, Pseudomonas aeruginosa
Catalog No : USB-215940
660.94€
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| Product name | Exotoxin A, Pseudomonas aeruginosa | ||
|---|---|---|---|
| Catalog No | USB-215940 | ||
| Supplier’s Catalog No | 215940 | ||
| Supplier | US Biologicals | ||
| Source antigen | Pseudomonas aeruginosa | ||
| Reactivity | |||
| Cross reactivity | |||
| Applications | |||
| Molecular weight | |||
| Storage | 4°C Do Not Freeze | ||
|---|---|---|---|
| Other names | |||
| Grade | Highly Purified | ||
| Purity | ≥95% (SDS-PAGE) | ||
| Form | Supplied as a lyophilized powder fom PBS, pH 7.5. Reconstitute with 500ul sterile ddH2O. Do not vortex. | ||
| Reactivity life | 12 months | ||
| Note | For reserch purpose only | ||
| Purity | ≥95% (SDS-PAGE) | ||
| Description | Exotoxin A is one of the toxic proteins released by pathogenic strains of Pseudomonas aeruginosa. It is secreted as a proenzyme with a molecular weight of 66kD. Like diphtheria toxin, exotoxin A is translocated into susceptible mammalian cells. Covalent alteration of the molecule occurs, rendering it enzymatically active. The intact toxin can be activated 20- to 50-fold in vitro by treatment with urea and dithiothreitol. Exotoxin A catalyzes the transfer of the adenosine diphosphate ribose moiety of oxidized nicotinamide adenine dinucleotide onto elongation factor 2(3): NAD + EF-2 ® ADP-ribose-EF-2 + nicotinamide + H+. This ADP-ribosylation of EF-2 blocks polypeptide assembly on the surface of the ribosome. As a result, this molecule is cytopathic for a number of cultured cell lines and is toxic to animals. Although exotoxin A and diphtheria toxin have identical enzymatic activity, they exhibit distinct target cell specificities and are immunologically unrelated. In crude preparations or in purified material which has been stored, lower molecular weight peptides with ADP-ribosylating activity may be present. Similarly, after reduction and limited proteolytic cleavage with chymotrypsin, a 26,000 dalton fragment that has enzymatic activity may be separated. This fragment alone is not toxic to intact cells. Source: Pseudomonas aeruginosa Applications: Suitable for use in immunodiffusion. Other applications not tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at 4°C Do Not Freeze. Reconstitute with sterile ddH2O. Aliquot and store at 4°C. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer. | ||
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