Toxin A, Clostridium Difficile
Catalog No : USB-215972
968.99€
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| Product name | Toxin A, Clostridium Difficile | ||
|---|---|---|---|
| Catalog No | USB-215972 | ||
| Supplier’s Catalog No | 215972 | ||
| Supplier | US Biologicals | ||
| Source antigen | Clostridium difficile | ||
| Reactivity | |||
| Cross reactivity | |||
| Applications | |||
| Molecular weight | 200 | ||
| Storage | 4°C Do Not Freeze | ||
|---|---|---|---|
| Other names | |||
| Grade | |||
| Purity | Single major band and several minor aggregate bands when run on a 3-8% gradient polyacrylamide-SOS gel. Endotoxin: ≤2EU/mg | ||
| Form | Supplied as a lyophilized powder from 50mM Tris, pH 7.5, 50mM sodium chloride, 0.1% trehalose. Reconstitute with 500ul sterile ddH2O. Handle Gently. Do not vortex. | ||
| Reactivity life | 12 months | ||
| Note | For reserch purpose only | ||
| Purity | Single major band and several minor aggregate bands when run on a 3-8% gradient polyacrylamide-SOS gel. Endotoxin: ≤2EU/mg | ||
| Description | A major nosocomial pathogen, Clostridium difficile causes antibiotic-associated colitis and intestinal Inflammatory disease, together called CDI. By releasing two large protein enterotoxins, Toxin A (TcdA) and Toxin B (TcdB), Clostridium difficile mediates inflammatory diarrhea and compromises intestinal epithelial cells. Either toxin when administered to cultured cells disrupts the cytoskeleton and sets off capsase-dependent apoptosis, although, TcdB is many times more potent than TcdA. Both toxins also activate intestinal epithelial and immune cells to produce cytokines and chemokines, likely contributing to the complications associated with severe CDI. Intracellular targets of TcdA and TcdB are RhoGTPases which regulate many host cell processes including establishing an epithelial barrier and the migration and signaling of immune cells. When the Rho proteins are glycosylated by Toxins A or B, signaling is disrupted leading to the intestinal damage and inflamation characteristic of CDI. Source: Toxin A from Clostridium difficile Molecular Weight: ~200kD Activity: Each lot of C. difficile toxin A is tested to confirm binding activity to fresh rabbit red blood cells using a hemagglutination assay; the results were acceptable. Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at 4°C Do Not Freeze. Reconstitute with sterile ddH2O. Aliquot and store at 4°C. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer. | ||
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