Toxin A, Clostridium Difficile
Catalog No : USB-215973
1162.10€
0.00€
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| Product name | Toxin A, Clostridium Difficile | ||
|---|---|---|---|
| Catalog No | USB-215973 | ||
| Supplier’s Catalog No | 215973 | ||
| Supplier | US Biologicals | ||
| Source antigen | Clostridium difficile | ||
| Reactivity | |||
| Cross reactivity | |||
| Applications | |||
| Molecular weight | |||
| Storage | 4°C Do Not Freeze | ||
|---|---|---|---|
| Other names | |||
| Grade | Molecular Biology Grade | ||
| Purity | |||
| Form | Supplied as a lyophilized powder in 0.1M histidine, 0.1M sodium chloride, pH 6.5, 5% trehalose. Reconstitute with 100ul sterile ddH2O. Handle Gently. Do not vortex. | ||
| Reactivity life | 12 months | ||
| Note | For reserch purpose only | ||
| Purity | |||
| Description | Clostridium difficile, or C. difficile, a gram positive anaerobic bacterium, is the major causative agent of colitis and diarrhea that can occur following antibiotic intake. C. difficile infection is one of the most common hospital acquired infections. When C. difficile colonize the gut, they release two potent toxins, toxin A and toxin B, which bind to certain receptors in the lining of the colon and ultimately cuase diarrhea and inflammation of the large intestine. Source: Toxin A Toxoid from Clostridium difficile Inactivation: Toxoid is produced from Toxin A of C. difficile by inactivation with formaldehyde. The resulting toxoid is essentially nontoxic. At 0.5mg/ml, the toxoid has no detectable cytotoxicity in a cell rounding assay using human foreskin cells, when compared to Toxin A. At the highest dose tested (50ug/mouse), mice injected intraperitoneally showed no toxic signs during the seven days of observation. Storage and Stability: Lyophilized powder may be stored at 4°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at 4°C. Reconstituted product is stable for 6 months at 4°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | ||
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