DENV, Recombinant, Dengue Virus 2, aa298-400, His-Tag (Dengue Virus 2 Envelope Protein)

Catalog No : USB-218102
564.38€
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Product name DENV, Recombinant, Dengue Virus 2, aa298-400, His-Tag (Dengue Virus 2 Envelope Protein)
Catalog No USB-218102
Supplier’s Catalog No 218102
Supplier US Biologicals
Source antigen Recombinant, E. coli
Reactivity
Cross reactivity
Applications
Molecular weight 13.8
Storage -20°C
Other names
Grade Highly Purified
Purity ~95% (SDS-PAGE)
Form Supplied as a liquid in 20mM Tris-HCl, pH 8.0, 0.15M sodium chloride, 1mM DTT, 10% glycerol.
Reactivity life 6 months
Note For reserch purpose only
Purity ~95% (SDS-PAGE)
Description Envelope protein E binding to host cell surface receptor is followed by virus internalization through clathrin-mediated endocytosis. Envelope protein E is subsequently involved in membrane fusion between virion and host late endosomes. This protein is synthesized as a homodimer with prM which acts as a chaperone for envelope protein E. After cleavage of prM, envelope protein E dissociate from small envelope protein M and homodimerizes. Source: Recombinant protein corresponding to aa298-400 from Dengue virus DENV, fused to His-tag at N-terminus, expressed in E.coli. Molecular Weight: ~13.8kD (126aa) Applications: Suitable for use in SDS-PAGE. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.