Cytomegalovirus pp65 (UL83), Recombinant, His-Tag (CMV pp65)

Catalog No : USB-298519
763.24€
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Product name Cytomegalovirus pp65 (UL83), Recombinant, His-Tag (CMV pp65)
Catalog No USB-298519
Supplier’s Catalog No 298519
Supplier US Biologicals
Source antigen Recombinant, E. coli
Reactivity
Cross reactivity
Applications
Molecular weight
Storage -20°C
Other names
Grade Highly Purified
Purity ~95% (SDS-PAGE)
Form Supplied as a liquid in 20mM sodium phosphate, 1M sodium chloride, 0.1% polyoxyethylene(10)tridecyl ether, 8M Urea, pH 8.0.
Reactivity life 12 months
Note For reserch purpose only
Purity ~95% (SDS-PAGE)
Description Cytomegalovirus is a member of the herpes virus group, which includes herpes simplex virus types 1 and 2, varicella zoster virus (which causes chicken pox), and Epstein Barr virus (which causes infectious mononucleosis). These viruses share a characteristic ability to remain dormant within the body over a long period. CMV viral genes are co-ordinately expressed in groups at various times after infection. Early viral proteins are expressed in the nucleus of infected cells within 3 to 24 hours of infection prior to the commencement of viral DNA replication. This is followed by expression of the early intermediate genes, which encode enzymes required for viral DNA replication. After 48 to 72 hours, a number of late viral antigens may be demonstrated in the nuclei and cytoplasm of infected cells. pp65 is a 65kD phosphorylated glycoprotein and is the most abundant of the late antigens. Source: Recombinant protein corresponding to CMV pp65, fused to His-tag at N-terminal expressed in E. coli. Applications: Suitable for use in ELISA, Western Blot. Other applications not tested. Recommended Dilution: ELISA: 0.78-2.2ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.