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Categories: Peptides & Proteins / Proteins

Product name			Treponema pallidum (Syphills), TpN47, Recombinant
Catalog No			USB-361724
Supplier’s Catalog No			361724
Supplier			US Biologicals
Source antigen			Recombinant, E. coli
Reactivity
Cross reactivity
Applications
Molecular weight			47.7

Storage			4°C
Other names
Grade			Highly Purified
Purity			~95% (SDS-PAGE)
Form			Supplied as a lyophilized powder in 20mM carbonate, pH 10.0. No preservative added.
Reactivity life			6 months
Note			For reserch purpose only
Purity			~95% (SDS-PAGE)
Description			The causative agent of Syphilis is a bacterium: Treponema pallidum. The immune responses to T. pallidum are primarily mediated by the following membrane lipoproteins: TpN47, TpN17, TpN15 and TmpA. TpN47 is highly immunogenic and activates endothelial cells, while TpN17 and TpN15 induce antibody responses. In independent studies, TmpA is the most frequently identified antigen (95%) while the remaining are recognized with the following prevalence: TpN47, 92.5%; TpN17, 89.5%; and TpN15, 67.5%. Source: Recombinant protein corresponding to Syphilis TpN47 expressed in E. coli. Molecular Weight: ~47.7kD Activity: Binds Syphilis antibodies Applications: Suitable for use in ELISA, Western Blot and Lateral Flow. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Reconstitute with sterile buffer or ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.