TmpA, Recombinant, T. Pallidum, His-Tag (Treponemal Membrane Protein A, Syphilis)

Catalog No : USB-367971
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Product name TmpA, Recombinant, T. Pallidum, His-Tag (Treponemal Membrane Protein A, Syphilis)
Catalog No USB-367971
Supplier’s Catalog No 367971
Supplier US Biologicals
Source antigen Recombinant, E. coli
Reactivity
Cross reactivity
Applications
Molecular weight 42.9
Storage -20°C/-70°C
Other names
Grade Highly Purified
Purity ~95% (SDS-PAGE)
Form Supplied as a liquid in 20mM phosphate, pH 8.0, 0.15M sodium chloride, 0.1% polyoxyethylene (10) tridecyl ether.
Reactivity life 12 months
Note For reserch purpose only
Purity ~95% (SDS-PAGE)
Description Treponema pallidum is the causative agent of syphilis. It is a spirochete, a helical to sinusoidal bacterium with outer and cytoplasmic membranes, a thin peptidoglycan layer, and periplasmic flagella. Mechanisms of T. pallidum pathogenesis are poorly understood. No known virulence factors have been identified, and the outer membrane is mostly lipid with a paucity of proteins. Consequently, existing diagnostic tests for syphilus are suboptimal, and no vaccine against T. pallidum is available. TIGR sequenced the genome of T. pallidum subsp. pallidum (strain Nichols) by the whole genome random sequencing method. The T. pallidum genome is a circular chromosome of 1,138,006 base pairs containing a total of 1041 predicted open reading frames. Source: Recombinant protein corresponding to mature TmpA without the leader peptide, fused to His-tag at N-terminal, expressed in E. coli. Molecular Weight: ~42.9kD Specificity: Reacts with human T. pallidum positive serum. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.