p47, Recombinant, T. pallidum, His-Tag

Catalog No : USB-397285
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Product name p47, Recombinant, T. pallidum, His-Tag
Catalog No USB-397285
Supplier’s Catalog No 397285
Supplier US Biologicals
Source antigen Recombinant, E. coli
Reactivity
Cross reactivity
Applications
Molecular weight 45
Storage -20°C
Other names
Grade Purified
Purity Purified
Form Supplied as a liquid in 150mM imidazole, pH 8.0, 150mM sodium chloride, 25mM sodium phosphate, 50% glycerol.
Reactivity life 6 months
Note For reserch purpose only
Purity Purified
Description Treponema pallidum is the causative agent of syphilis. It is a spirochete, a helical to sinusoidal bacterium with outer and cytoplasmic membranes, a thin peptidoglycan layer, and periplasmic flagella. Mechanisms of T. pallidum pathogenesis are poorly understood. No known virulence factors have been identified, and the outer membrane is mostly lipid with a paucity of proteins. Consequently, existing diagnostic tests for syphilus are suboptimal, and no vaccine against T. pallidum is available. TIGR sequenced the genome of T. pallidum subsp. pallidum (strain Nichols) by the whole genome random sequencing method. The T. pallidum genome is a circular chromosome of 1,138,006 base pairs containing a total of 1041 predicted open reading frames. Source: Recombinant protein corresponding to T. pallidum p47, fused to His-tag, expressed in E. coli. Molecular Weight: ~45kD Applications: Suitable for use in ELISA. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.