Epstein-Barr Viral Capsid Antigen (EBV-VCA gp125)
Catalog No : USB-407358
542.54€
0.00€
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| Product name | Epstein-Barr Viral Capsid Antigen (EBV-VCA gp125) | ||
|---|---|---|---|
| Catalog No | USB-407358 | ||
| Supplier’s Catalog No | 407358 | ||
| Supplier | US Biologicals | ||
| Source antigen | P3H3 cells chronically infected with EBV. | ||
| Reactivity | |||
| Cross reactivity | |||
| Applications | |||
| Molecular weight | |||
| Storage | -70°C | ||
|---|---|---|---|
| Other names | |||
| Grade | Highly Purified | ||
| Purity | ~99%. Chronically infected cells are harvested by centrifugation and disrupted by sonication and cell debris is removed by centrifugation. This lysate is purified on an immunoaffinity column using a monoclonal antibody against gp125. | ||
| Form | Supplied as a liquid in 20mM Tris-HCl, pH 7.4, 3M MgCl2. | ||
| Reactivity life | 6 months | ||
| Note | For reserch purpose only | ||
| Purity | ~99%. Chronically infected cells are harvested by centrifugation and disrupted by sonication and cell debris is removed by centrifugation. This lysate is purified on an immunoaffinity column using a monoclonal antibody against gp125. | ||
| Description | This preparation contains more than 90% virus specific protein. The predominant form of the antigen is whole virions suspended in NTE with sucrose. The preparation is free of detergent. Residual non-viral protein comes from host tissue and culture medium. Source: Highly purified (>99%) 125kD protein from P3H3 cells chronically infected with EBV. Applications: Suitable for use in a variety of immunoassay formats including micro well ELISA. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Inactivation: Detergent inactivation by lysis. Detergent treatment of P3H3 cells disrupts cellular and viral membranes and consequently renders the material non-infectious. Storage and Stability: Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | ||
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