Hepatitis A, HM-175 Strain

Catalog No : USB-407359
425.30€
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Product name Hepatitis A, HM-175 Strain
Catalog No USB-407359
Supplier’s Catalog No 407359
Supplier US Biologicals
Source antigen FRhK4
Reactivity
Cross reactivity
Applications
Molecular weight
Storage -70°C
Other names
Grade Purified
Purity Cells which have been infected with HAV are collected by centrifugation. Pelleted cells are resuspended in glycine buffer and the cells are disrupted releasing the viral particles. Cellular debris is removed by centrifugation and the antigen preparation is further purified by extraction with Galden Perfluorosolv. The final concentration of HAV antigen is adjusted to meet specifications by dilution.
Form Supplied as a liquid in glycine, pH 9.5.
Reactivity life 6 months
Note For reserch purpose only
Purity Cells which have been infected with HAV are collected by centrifugation. Pelleted cells are resuspended in glycine buffer and the cells are disrupted releasing the viral particles. Cellular debris is removed by centrifugation and the antigen preparation is further purified by extraction with Galden Perfluorosolv. The final concentration of HAV antigen is adjusted to meet specifications by dilution.
Description Hepatitis A virus is a non-enveloped, icosahedral virion with a single stranded RNA genome. Only one serotype of HAV is known and it does not cross react with Hepatitis B virus. The virus contains four proteins VP1, VP2, VP3 and VP4. Source: Hepatitis A, strain HM-175, from FRhK4, an established cell line from fetal Rhesus Monkey kidney. Applications: Suitable for use in ELISA. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Inactivation: HAV grade 2 Antigen is inactivated by incubating with formaldehyde for 96 hours. Storage and Stability: Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.