Mumps grade 2, Enders Strain
Catalog No : USB-407364
462.08€
0.00€
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| Product name | Mumps grade 2, Enders Strain | ||
|---|---|---|---|
| Catalog No | USB-407364 | ||
| Supplier’s Catalog No | 407364 | ||
| Supplier | US Biologicals | ||
| Source antigen | BSC-1 cells | ||
| Reactivity | |||
| Cross reactivity | |||
| Applications | |||
| Molecular weight | |||
| Storage | -70°C | ||
|---|---|---|---|
| Other names | |||
| Grade | Purified | ||
| Purity | Optimally infected cells are harvested, disrupted by sonication in culture medium and then subjected to low speed centrifugation. The supernatant from the infected culture is concentrated using crossflow ultrafiltration. | ||
| Form | Supplied as a liquid in 1969B. | ||
| Reactivity life | 6 months | ||
| Note | For reserch purpose only | ||
| Purity | Optimally infected cells are harvested, disrupted by sonication in culture medium and then subjected to low speed centrifugation. The supernatant from the infected culture is concentrated using crossflow ultrafiltration. | ||
| Description | The resulting antigen preparation contains a high concentration of virus and viral components as well as some cellular material suspended in 1969B. Mumps virus is a member of the Paramyxoviridae family. There is only one serotype. The virion is pleomorphic with a roughly spherical shape and a diameter of 85 to 300 µm. The nucleocapsid is composed of a continuous linear molecule of single-stranded RNA that is tightly coiled in a helical formation and a capsid protein with RNA polymerase activity. The nucleocapsid is surrounded by a lipid envelope composed of three layers. The inner surface is a protein that supports the outer layers, the middle is a lipid bilayer acquired as the virion buds from the host cell, and the external layer is studded with glycoproteins that carry receptordestroying, hemagglutinating and cell fusion activity. The hemagglutinating protein binds to sialic acid residues on target cells to initiate infection. The cell fusion protein is responsible for the fusion of lipid bilayers that enables penetration of the virus and cell-to-cell spread of infection. Source: Mumps, strain Enders, from BSC-1 cells. Applications: Suitable for use in both IgG and IgM detection in assays which include EIA with polystyrene and latex solid phases. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Inactivation: Mumps antigen is inactivated using gamma radiation. This procedure is effective primarily by damaging viral genetic material. Storage and Stability: Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | ||
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