Toxoplasma gondii, IgM Grade, RH Strain
Catalog No : USB-407375
405.76€
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| Product name | Toxoplasma gondii, IgM Grade, RH Strain | ||
|---|---|---|---|
| Catalog No | USB-407375 | ||
| Supplier’s Catalog No | 407375 | ||
| Supplier | US Biologicals | ||
| Source antigen | Vero cells | ||
| Reactivity | |||
| Cross reactivity | |||
| Applications | |||
| Molecular weight | |||
| Storage | -70°C | ||
|---|---|---|---|
| Other names | |||
| Grade | Purified | ||
| Purity | Confluent cultures of Vero cells are infected with Toxoplasma and the infection is allowed to proceed. Cells are harvested and disrupted to harvest tachyzoites, which are washed and the concentration adjusted. Tachyzoities are then disrupted and purified using a method designed to enrich membrane fractions, including the apical complex which is thought to be associated with active motility during parasite invasion and is reactive with IgM antibodies. The material is inactivated and standardized based on the ELISA results compared to a reference antigen. This antigen preparation contains a high concentration of disrupted tachyzoites in phosphate buffered saline. | ||
| Form | Supplied as a liquid in 2% N-octyl-beta-D-glucopyranoside (OGP), PBS, pH 7.4. | ||
| Reactivity life | 6 months | ||
| Note | For reserch purpose only | ||
| Purity | Confluent cultures of Vero cells are infected with Toxoplasma and the infection is allowed to proceed. Cells are harvested and disrupted to harvest tachyzoites, which are washed and the concentration adjusted. Tachyzoities are then disrupted and purified using a method designed to enrich membrane fractions, including the apical complex which is thought to be associated with active motility during parasite invasion and is reactive with IgM antibodies. The material is inactivated and standardized based on the ELISA results compared to a reference antigen. This antigen preparation contains a high concentration of disrupted tachyzoites in phosphate buffered saline. | ||
| Description | Toxoplasma in healthy individuals is most often asymptomatic. When symptomatic disease occurs it often resembles mononucleosis or may be limited to an ocular infection. Subclinical infections may surface some years after infection when the individual becomes immunocompromised. In these patients the result is often cerebral infection. Congenital infections are the primary area of concern as the symptoms may involve severe defects of the central nervous system and eyes which is often fatal. Source: Toxoplasma, strain RH, from Vero cells. Applications: Suitable for use in a variety of immunoassay formats where high purity Toxoplasma antigens are required, particularly when clear differentiation is required between IgM positive and IgM negative sera. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Inactivation: Detergent treatment. Storage and Stability: Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | ||
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