HIV-1 [HIV-1/Clade B (CN54)] GP120, Recombinant (Human Immunodeficiency Virus-1)
Catalog No : USB-506308
857.50€
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| Product name | HIV-1 [HIV-1/Clade B (CN54)] GP120, Recombinant (Human Immunodeficiency Virus-1) | ||
|---|---|---|---|
| Catalog No | USB-506308 | ||
| Supplier’s Catalog No | 506308 | ||
| Supplier | US Biologicals | ||
| Source antigen | Recombinant, HEK293 cells | ||
| Reactivity | |||
| Cross reactivity | |||
| Applications | |||
| Molecular weight | 53.8 | ||
| Storage | -20°C | ||
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| Other names | |||
| Grade | Purified | ||
| Purity | ~90% (SDS-PAGE) | ||
| Form | Supplied as a lyophilized powder in DPBS, pH 7.4. Reconstitute with 500ul sterile ddH2O. | ||
| Reactivity life | 6 months | ||
| Note | For reserch purpose only | ||
| Purity | ~90% (SDS-PAGE) | ||
| Description | HIV-1 viruses may be further divided into groups, being M, N, O and P. The HIV-1 group M viruses predominate and are responsible for the AIDS pandemic. Within the M group of HIV-1 there are a number of genetically distinct sub-types (also known as clades). Different subtypes can also combine genetic material to form a hybrid virus or “circulating recombinant form (CRF)”. Subtype B is the most common in the Americas and Western Europe, whilst subtype C is the predominant form in Africa and India. Most research has been carried out into subtype B, although it accounts for only around 12% of infections worldwide. HIV GP120 protein (or gp120) is the name of the glycoprotein which forms the spikes sticking out of a HIV virus particle. HIV gp120 protein is essential for virus entry into cells as it plays a vital role in seeking out specific cell surface receptors for entry. Three gp120s, bound as heterodimers to a transmembrane glycoprotein, gp41, are thought to combine in a trimer to form the envelope spike, which is involved in virus-cell attachment. Approximately 50% of the mass of HIV gp120 protein is due to glycosylation, the high level of which may prevent gp120 from being recognized by the human immune response. gp120 binds to the human cell surface antigen CD4, which is primarily expressed by Helper T lymphocytes and monocytes/macrophages. This recombinant HIV gp120 protein is derived from a CRF first isolated in China, which is a combination of subtypes B and C. It is derived from the env. gene of HIV-1 strain CN54 gp160, fused with a 6xHis-Tag at the C-terminal, and has a calculated MW of 53.8kD. The protein migrates at 80-110kD in reducing SDS-PAGE due to glycosylation Source: Recombinant protein corresponding to Thr27-Arg498 of HIV-1 GP120 (HIV-1/Clade B/C (CN54)), derived from the env. gene of HIV-1 strain CN54 gp160, and glycosylated with N-linked sugars, expressed in HEK293 cells Molecular Weight: ~53.8kD, migrates at ~80-110kD in reducing SDS-PAGE due to glycosylation Applications: Has been shown to bind to CD4 in a ELISA assay format. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O or PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | ||
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