Insulin Regulated Aminopeptidase, Rat, Control Peptide (IRAP, Placental Leucine Aminopeptidase, PLAP, Leucyl Cystinyl Aminopeptidase, LNPEP, Oxytocinase, Otase, Vesicle Protein of 165kD, vp165)
Catalog No : USB-I8601-01A
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| Product name | Insulin Regulated Aminopeptidase, Rat, Control Peptide (IRAP, Placental Leucine Aminopeptidase, PLAP, Leucyl Cystinyl Aminopeptidase, LNPEP, Oxytocinase, Otase, Vesicle Protein of 165kD, vp165) | ||
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| Catalog No | USB-I8601-01A | ||
| Supplier’s Catalog No | I8601-01A | ||
| Supplier | US Biologicals | ||
| Source antigen | Rat synthetic peptide | ||
| Reactivity | |||
| Cross reactivity | Human, Mouse, | ||
| Applications | |||
| Molecular weight | |||
| Storage | -20°C | ||
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| Other names | |||
| Grade | Highly Purified | ||
| Purity | Highly purified | ||
| Form | Supplied as a liquid in PBS, pH 7.2 | ||
| Reactivity life | 12 months | ||
| Note | For reserch purpose only | ||
| Purity | Highly purified | ||
| Description | A 17-aa Peptide ~900 aa, extracellular domain of rat IRAP. Control peptide sequence is 82% conserved in mouse and 76% in human IRAP isoform 1 and 2. Control Peptide for I8601 (antiserum) and I8601-01 (affinity purified) Tankyrases (TRF1 interacting ankyrin-related ADP-ribose polymerase; human 1327 aa, renamed as TNKS-1/TANK1, chromosome 8, and its homolog TANK2), a modular protein with homology to ankyrin and poly(adenosine diphosphate-ribose) polymerase (PARP) has been cloned and localized to telomere. In addition to regulating the telomere and nuclear localization, TANKs also resides at the other subcellular compartments such as nuclear envelope, specifically on cytoplasmic fibers of nuclear pore complexes. TANK1 is targeted to pericentriolar domain of centrosomes during mitosis. After mitosis, TANK1 associates with the golgi as a peripheral membrane protein and implicated in targeting of GLUT4 vesicles. In the absence of insulin, GLUT4 vesicles reside in the Golgi and cytoplasm. Upon stimulation by insulin, GLUT4 vesicles undergo exocytosis and translocate to the plasma membrane. GLUT4 vesicles are characterized by the presence of glucose transporter 4 (GLUT4) and IRAP (Insulin regulated aminopeptidase). The exocytosis of GLUT4 vesicles is therefore plays an important role in uptake of glucose through Glut4 and the degradation of various vascoactive hormones by IRAP. TANKs have been colocalized in GLUT4 vesicles and shown to bind IRAP via the ANK-repeats. IRAP (also known as placental leucine aminopeptidase, PLAP; leucyl cystinyl aminopeptidase, LNPEP; oxytocinase, Otase; vesicle protein of 165kD or vp165) belongs to the peptidase M1 family. IRAP (mouse 966aa, rat 1025 aa; human 1025 aa, chromosome 5q14.2-q15) release an n-terminal amino acid, cleave before cysteine, leucine as well as other amino acids. IRAP, a zinc-containing metalloprotein, degrade peptide hormone such as oxytocin, vasopressin, and angiotensin III, inactivate Met-enkephalin and dynorphin. It is a type 2 membrane protein and also secreted due to proteolytic processing. IRAP is glycosylated and alternatively spliced to at least 3 isoform (1-15 aa missing in isoform 2 and 1-20 missing in isoform 3). The N-terminus (1-110 aa) is located in the cytoplasm, followed by TM domain (111-131 aa) and the C-terminus (132-1025 aa) is extracellualr. The secreted form is cleaved at 154-155 aa (processed secreted form 155-1025 aa). IRAP is highly expressed in placenta, heart, kidney, and small intestine. In brain, only the membrane form is found. The brain form is found to be 140kD due to differential glycosylation. Applications: Suitable for use in ELISA and Antibody. Blocking. Control peptide, because of its low mol. Wt (<3 kD), is not suitable for Western Blot.Other applications not tested. Recommended Dilution: ELISA: Control peptide can be used to coat ELISA plates at 1ug/ml. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | ||
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