Insulin Receptor, beta, Recombinant, Human, GST-Tag (IRb)

Catalog No : USB-I7661-26K
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Product name Insulin Receptor, beta, Recombinant, Human, GST-Tag (IRb)
Catalog No USB-I7661-26K
Supplier’s Catalog No I7661-26K
Supplier US Biologicals
Source antigen Recombinant, Baculovirus infected Sf9 cells
Reactivity
Cross reactivity
Applications
Molecular weight 71.7
Storage -70°C
Other names
Grade Purified
Purity ≥ 80% by SDS-PAGE
Form Supplied as a liquid in 50mM Tris, pH 7.5 and 1mM DTT.
Reactivity life 6 months
Note For reserch purpose only
Purity ≥ 80% by SDS-PAGE
Description Human INSR, Insulin receptor (beta) (GenBank Accession No. NM_000208). GST fusion protein containing the Mr 48kD cytoplasmic domain of the b-subunit of the human insulin receptor, residues 941-1343, expressed in a baculovirus expression system. Specific Activity: ~117 nmol/min/mg @ 25°C using poly(Glu:Tyr) 4:1 substrate. Reaction conditions are 50mM Tris, pH 7.5, 30mM MgCl2, 1 mM DTT, 1.0mM ATP and 1mg/ml substrate. Applications: Suitable for use in Enzyme kinetics, autophosphorylation, and phosphorylation of target substrates. Other applications not tested. Storage and Stability: Aliquot to avoid repeated freezing and thawing and store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 6 months.