Integrin alphaM, beta2, Recombinant, Human (Mac-1, CD11b/CD18)
Catalog No : USB-I7661-37Y1
740.25€
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| Product name | Integrin alphaM, beta2, Recombinant, Human (Mac-1, CD11b/CD18) | ||
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| Catalog No | USB-I7661-37Y1 | ||
| Supplier’s Catalog No | I7661-37Y1 | ||
| Supplier | US Biologicals | ||
| Source antigen | Recombinant, Chinese hamster ovary (CHO) cells | ||
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| Cross reactivity | |||
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| Molecular weight | |||
| Storage | -20°C | ||
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| Grade | Highly Purified | ||
| Purity | ~95%, as determined by SDS-PAGE under reducing conditions and visualized by silver stain. Endotoxin: ≤ 1EU /ug (LAL). | ||
| Form | Supplied as lyophilized powder from 50mM Tris, 0.15M sodium chloride, 1mM MgCl2, pH 7.4. Reconstitute with sterile PBS to 0.1mg/ml. | ||
| Reactivity life | 6 months | ||
| Note | For reserch purpose only | ||
| Purity | ~95%, as determined by SDS-PAGE under reducing conditions and visualized by silver stain. Endotoxin: ≤ 1EU /ug (LAL). | ||
| Description | DNA sequences encoding the extracellular domain of human Integrin alpha M (Phe 17-Asn 1104; Accession# P11215) (Corbi, A.L., et al., 1988, J. Biol. Chem. 263:12403) with an acid tail at the C-terminus and the extracellular domain of human Integrin b2 (Gln 22-Asn 700; Accession# P05107) (Kishimoto, T. et al., 1987, Cell 48:681) with a basic tail at the C-terminus were cloned downstream of the human CD33 signal peptides and co-expressed in Chinese hamster ovary (CHO) cells. Specifictity: The 1088aa human aM/CD11b ECD shares 73-76% aa sequence identity with mouse, rat, bovine and canine aM while the 678aa human b2/CD18 ECD shares 81-83% aa sequence identity with mouse, rat, bovine, canine, goat, sheep, and porcine b2. Molecular Mass: Based on N-terminal sequencing, the recombinant human Integrin aM subunit starts at Phe 17 and has a calculated molecular mass of ~124.7kD. The N-terminal sequence of the b2 subunit was blocked, suggesting that the b2 subunit may start at the predicted Gln 23 and has a calculated molecular mass of a~79kD. As a result of glycosylation, the recombinant proteins migrate as ~165 and 100kD protein bands in SDS-PAGE under reducing conditions. Activity: Measured by the ability of the immobilized protein to support the adhesion of CHO cells transfected with human ICAM-1. When 5x10e4 cells/well are added to Integrin aMb2-coated plates (5ug/ml, 100ul/well), 70-90% of the cells will adhere after 1 hour at 37°C in the presence of 1mM MnCl2. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot and store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | ||
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