ITGA11, ITGB1, Recombinant, Human (Integrin alpha 11 beta 1, Integrin alpha-11, MSTP018 / Integrin beta-1, Fibronectin Receptor Subunit beta, VLA-4 Subunit beta, CD29, FNRB, MDF2, MSK12)
Catalog No : USB-I7661-37H
717.26€
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| Product name | ITGA11, ITGB1, Recombinant, Human (Integrin alpha 11 beta 1, Integrin alpha-11, MSTP018 / Integrin beta-1, Fibronectin Receptor Subunit beta, VLA-4 Subunit beta, CD29, FNRB, MDF2, MSK12) | ||
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| Catalog No | USB-I7661-37H | ||
| Supplier’s Catalog No | I7661-37H | ||
| Supplier | US Biologicals | ||
| Source antigen | Recombinant, CHO cells | ||
| Reactivity | |||
| Cross reactivity | |||
| Applications | |||
| Molecular weight | 134.6118841 | ||
| Storage | -20°C | ||
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| Other names | |||
| Grade | Highly Purified | ||
| Purity | ~95% (SDS-PAGE) | ||
| Form | Supplied as a lyophilized powder from PBS, pH 7.4. Reconstitute with 500ul sterile PBS. | ||
| Reactivity life | 6 months | ||
| Note | For reserch purpose only | ||
| Purity | ~95% (SDS-PAGE) | ||
| Description | Integrin a11B1 is a noncovalent, heterdimeric collagen receptor that contains a 150kD a11 subunit complexed to a 130kD B1/CD29 subunit. a11B1 is the only known a11-containing integrin. By contrast, at least twelve a-integrins share the B1 subunit. a11B1 is expressed on fibroblasts from cornea, skin and periodontal ligament (3-9). It is also expressed on mesenchymal stem cells and is considered a marker for non-muscle mesenchymal tissue (3, 8-10). Expression of a11B1 is stimulated by TGF-B1, activin A, and type I interferons, and down-regulated by FGF-2. The a11 extracellular domain (ECD) contains a vWFA domain which includes the ligand binding site. The B1 ECD also contains a vWFA domain, which participates in binding. Each subunit has a transmembrane sequence and a short cytoplasmic tail. The dimer assumes a folded configuration at "rest". Divalent cations and intracellular (inside-out) signaling convert it to an active, extended and open conformation. The 1120aa human a11 ECD shares 90-93% aa sequence identity with mouse, rat, equine, canine and bovine a11, while the 708aa human B1 ECD shares 92-96% aa sequence identity with rat, bovine, mouse, and feline B1. I domain-containing B1 integrins a1B1, a2B1, a10B1 and a11B1 all bind collagens. a11B1 preferentially binds interstitial collagens such as type I. It can contract collagen matrices and influence inflammatory edema. Depending on context, a11B1 can support or inhibit cell migration on collagen. It is frequently up-regulated in the stroma of lung adenocarcinomas and enhances tumorigenicity Recombinant non-covalent heterodimer corresponding to aa23-1141 of human Integrin a11, acidic tail at N-terminal and HHHHHH at C-terminal Accession # (Q9UKX5) and aa21-728 of human Integrin B1, basic tail at N-terminal Accession # (P05556). Molecular Weight: ~134.6kD (a11), ~88.4kD (B1) Endotoxin Level: ≤0.1EU/1ug (LAL) Biological Activity: Measured by its binding ability in a functional ELISA. Immobilized Collagen I at 10ug/ml (100ul/well) can bind recombinant human Integrin a11B1 with an apparent KD<2nM. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 6 months at -20°C. Reconstitute with PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | ||
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