Integrin alpha 6 beta 1, Recombinant, Mouse, aa21-1011 (Integrin alpha6beta1, Platelet Glycoprotein GPIcIIa)
Catalog No : USB-145811
682.78€
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| Product name | Integrin alpha 6 beta 1, Recombinant, Mouse, aa21-1011 (Integrin alpha6beta1, Platelet Glycoprotein GPIcIIa) | ||
|---|---|---|---|
| Catalog No | USB-145811 | ||
| Supplier’s Catalog No | 145811 | ||
| Supplier | US Biologicals | ||
| Source antigen | Recombinant | ||
| Reactivity | |||
| Cross reactivity | |||
| Applications | |||
| Molecular weight | 105120125150 | ||
| Storage | -20°C | ||
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| Other names | |||
| Grade | Highly Purified | ||
| Purity | ~95% (SDSPAGE) | ||
| Form | Supplied as a lyophilized powder in PBS. BSA free. Reconstitute in 250ug/ml PBS. | ||
| Reactivity life | 6 months | ||
| Note | For reserch purpose only | ||
| Purity | ~95% (SDSPAGE) | ||
| Description | Integrin alpha6beta1, also called platelet glycoprotein GPIcIIa, is a laminin binding integrin that is expressed on T cells, monocytes, endothelial cells, stem cells, and platelets (19). The noncovalent heterodimer is composed of ~150kD alpha6/CD49f and 130kD beta1/CD29 type I transmembrane glycoprotein subunits (2). While alpha6 pairs only with beta1 or beta4, twelve integrins share the beta1 subunit (15). The alpha6 subunit is cleaved into extracellular heavy and transmembrane light chains (3). Alternative splicing in the human alpha6 extracellular domain (ECD) at amino acid (aa) 216 creates X1 (ubiquitous), X2 and X1X2 isoforms, while splicing at a mouse or human cytoplasmic site creates A and B isoforms (10, 11). These forms do not appear to alter the binding specificity (4, 10, 11). The beta1 ECD contains a vWFA domain, which participates in binding. Each subunit then has a transmembrane sequence and a short cytoplasmic tail. The dimer is folded when it is least active. Divalent cations and intracellular (insideout) signaling convert it to its most active, extended and open conformation (1, 2). The mouse alpha6 heavy chain shares 98% aa identity with rat and 9293% with human (X1), bovine, and canine alpha6, and the mouse beta1 ECD shares 98% aa identity with rat and 9394% with human, bovine, porcine, ovine, canine and feline beta1. alpha6beta1 shows broad specificity for adhesion to laminin isoforms (4, 10). Its expression on human and mouse pluripotent stem cells is important for attachment, expansion, and selfrenewal on LN511 (laminin alpha5beta1gamma1) (6, 7). The secreted protein Netrin4 and the laminin gamma1 subunit form an adhesionactivating complex with alpha6beta1 on mouse neural stem cells and human lymphatic endothelial cells that promotes lymphangiogenesis (8, 9). alpha6beta1 upregulation on cancers such as prostate, glioma, and hepatoma is reported to enhance tumorigenicity, motility, invasion and metastasis (1214). alpha6beta1 cleavage via uPA (urokinasetype plasminogen activator) facilitates tumorigenicity in prostate cancers, and interaction of hepatoma alpha6beta1 with EMMPRIN/CD147 may also enhance tumorigenicity by inducing uPA and other metalloproteinases (12, 13). Source: Recombinant protein corresponding to aa21-1011 from mouse Integrin alpha 6 beta 1, CHO-derived. Molecular Weight: ~105-120kD Endotoxin: <0.10EU/1ug (LAL method) Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute in sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | ||
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