Ferroportin (FPN) Recombinant, Human
Catalog No : USB-154536
401.16€
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| Product name | Ferroportin (FPN) Recombinant, Human | ||
|---|---|---|---|
| Catalog No | USB-154536 | ||
| Supplier’s Catalog No | 154536 | ||
| Supplier | US Biologicals | ||
| Source antigen | E. coli | ||
| Reactivity | |||
| Cross reactivity | |||
| Applications | |||
| Molecular weight | 52 | ||
| Storage | -70°C/-20°C | ||
|---|---|---|---|
| Other names | |||
| Grade | Purified | ||
| Purity | ≥80% | ||
| Form | Supplied as lyophilized powder from 20mM Tris, 150mM sodium chloride, pH 8.0, 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, Proclin-300. Reconstitute with 20mM Tris, 150mM sodium chloride, pH 8.0 to a concentration of 0.1-1mg/ml. Do not vortex. | ||
| Reactivity life | 6 months | ||
| Note | For reserch purpose only | ||
| Purity | ≥80% | ||
| Description | Recombinant protein corresponding to Trp127-Val321 linked with LEHHHHHH with two N-Terminal Tags, His-Tag and GST-Tag, from human FPN, expressed in E. coli (Q9NP59). Amino Acid Sequence: WVLTSCYILI ITIANIANLA STATAITIQR DWIVVVAGED RSKLANMNAT IRRIDQLTNI LAPMAVGQIM TFGSPVIGCG FISGWNLVSM CVEYVLLWKV YQKTPALAVK AGLKEEETEL KQLNLHKDTE PKPLEGTHLM GVKDSNIHEL EHEQEPTCAS QMAEPFRTFR DGWVSYYNQP VFLAGMGLAF LYMTV Predicted Molecular Weight: 52.8kD Accurate Molecular Mass: 52kD as determined by SDS-PAGE under reducing conditions Predicted Isoelectric Point: 6.0 Subcellular Location: Cell membrane; Multi-pass membrane protein Tissue Specificity: Placenta, Spleen Applications: Suitable for use in Western Blot and SDS-PAGE. Other applications not tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Included Protein Molecular Weight Marker: 168631: Unstained Protein Molecular Weight Marker, 10-70kD Suppled as a liquid in 62.5mM Tris-H3PO4, pH 7.5, 1mM EDTA, 2% SDS, 100mM DTT, 1mM sodium azide, 0.01% bromo-phenol blue, 33% glycerol. Ready to use; no need to heat, dilute or add reducing agents before use. Protein Bands: 10kD, 14kD, 18kD, 22kD, 26kD, 33kD, 44kD and 70kD. Double Intesity Bands: The 26kD, 18kD and 10kD bands are at double intensity to make location and size approximation of proteins easier. Usage: Allow the marker to reach room temperature and mix thoroughly before use to ensure that any solids that may have precipitated at -20°C will go back into solution. Do not boil! Load the following volumes on SDS-PAGE gel: Mini gels: 3-5ul/well Large gel: 7ul/well The loading volumes listed above are recommended for gels with thickness of 0.75-1mm. The loading volume should be doubled for 1.5mm thick gels. Store at -20°C Stable for 6 months after receipt. Storage and Stability: Lyophilized powder may be stored at -70°C. Stable for 6 months after receipt. Reconstitute with sterile 20mM Tris, 150mM sodium chloride, pH 8.0. Aliquot to avoid repeated freezing and thawing. Store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Note: Thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation by incubating the protein at 37°C for 48h, with no obvious degradation or precipitation observed. Protein loss is <5% within the expiration date under appropriate storage conditions. | ||
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