Cytokeratin 8, Recombinant, Human
Catalog No : USB-C9097-42R
377.02€
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| Product name | Cytokeratin 8, Recombinant, Human | ||
|---|---|---|---|
| Catalog No | USB-C9097-42R | ||
| Supplier’s Catalog No | C9097-42R | ||
| Supplier | US Biologicals | ||
| Source antigen | Recombinant, E. coli | ||
| Reactivity | |||
| Cross reactivity | |||
| Applications | |||
| Molecular weight | |||
| Storage | -20°C | ||
|---|---|---|---|
| Other names | |||
| Grade | Highly Purified | ||
| Purity | > 95% by RP-HPLC, FPLC, or reducing/non-reducing SDS-PAGE Silver Stain. Chromatographically purified. Endotoxin: ≤ 0.1ng/ug (IEU/ug). | ||
| Form | Supplied as a lyophilized powder from 30mM Tris-HCl, pH 8.0, 9.5M urea, 2mM DTT, 2mM EDTA, 10mM methylammonium chloride. Reconstitute the lyophilized CK-18 in sterile ddH2O, 0.1% BSA or HSA to > 0.1mg/ml, which can then be further diluted to other aqueous solutions. | ||
| Reactivity life | 6 months | ||
| Note | For reserch purpose only | ||
| Purity | > 95% by RP-HPLC, FPLC, or reducing/non-reducing SDS-PAGE Silver Stain. Chromatographically purified. Endotoxin: ≤ 0.1ng/ug (IEU/ug). | ||
| Description | Keratins are a class of fibrous proteins or scleroproteins important both as structural proteins and as keys to the study of protein conformation. The family represents the principal constituent of epidermis, hair, nails, horny tissues, and the organic matrix of tooth enamel. Two major conformational groups have been characterized, alpha-keratin, whose peptide backbone forms an alpha-helix, and beta-keratin, whose backbone forms a zigzag or pleated sheet structure. Keratin 8 is a major component of intermediate filaments in single-layered epithelia of the gastrointestinal tract. Keratin 8 deficient mice display signs of colitis and diarrhoea characteristic for inflammatory bowel disease. Recombinant Human Cytokeratin 8 is a single, non-glycosylated polypeptide chain having a molecular mass of 53.53kD Dimers and Aggregates: ≤ 1% as determined by silver-stained SDS-PAGE gel analysis. Protein Content: Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280nm. 2. Analysis by RP-HPLC, using a calibrated solution of cytokeratin 8 as a Reference Standard. Storage and Stability: Lyophilized powder may be stored at -20°C. Reconstitute to nominal volume by adding sterile dH2O, 0.1% BSA or HSA. Aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | ||
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