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Categories: Peptides & Proteins / Proteins

Product name			KRT18, Recombinant, Human, Western Blot Control (Keratin, Type I Cytoskeletal 18, Cytokeratin-18, CK-18, Keratin-18, K18, Cell Proliferation-inducing Gene 46 Protein, PIG46, CYK18)
Catalog No			USB-K0199-22P
Supplier’s Catalog No			K0199-22P
Supplier			US Biologicals
Source antigen			Recombinant, E. coli
Reactivity
Cross reactivity
Applications
Molecular weight			51.6

Storage			-20°C
Other names
Grade			Purified
Purity			Purified (SDS-PAGE)
Form			Supplied as a liquid in 25mM Tris-HCl, pH6.8, 50mM DTT, 1% SDS, 0.1% Bromophenol Blue, 2.5% glycerol.
Reactivity life			6 months
Note			For reserch purpose only
Purity			Purified (SDS-PAGE)
Description			Tissue polypeptide specific antigen was first identified as a tumor marker in the late 1950’s, but has recently been shown to be closely related to human cytokeratin 18. Cytokeratins are a group of about 20 cytoskeletal proteins, whose degradation gives rise to soluble fragments, measurable in the blood of patients. Serum levels are high in breast cancer patients and also some other malignancies, probably due to high cell turnover in tumors. Source: Recombinant corresponding to human CK-18, fused to 6x His-tag at N-terminal, expressed in E. coli. Molecular Weight: ~51.6kD Applications: Suitable for use in Western Blot as a control. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.