Datura stramonium (DSA/DSL)
Catalog No : USB-518303
374.72€
0.00€
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| Product name | Datura stramonium (DSA/DSL) | ||
|---|---|---|---|
| Catalog No | USB-518303 | ||
| Supplier’s Catalog No | 518303 | ||
| Supplier | US Biologicals | ||
| Source antigen | Jimson weed | ||
| Reactivity | |||
| Cross reactivity | |||
| Applications | |||
| Molecular weight | 86 | ||
| Storage | -20°C | ||
|---|---|---|---|
| Other names | |||
| Grade | Affinity purified | ||
| Purity | Affinity purified | ||
| Form | Supplied as a lyophilized powder. | ||
| Reactivity life | 2 years | ||
| Note | For reserch purpose only | ||
| Purity | Affinity purified | ||
| Description | Datura stramonium agglutinin or lectin (DSA/DSL) is purified by affinity chromatography. Seeds of Datura stramonium contain at least three different carbohydrate-binding proteins, and the most prominent lectin is a dimeric glycoprotein composed of two nonidentical subunits that contains 40% carbohydrate. It binds well to N-acetylglucosamine, oligomers, and branched pentasaccharide, including two N-acetylglucosamine disaccharides linked to mannose (β-1,6) and (β-1,2), which is known to be the most potent inhibitor of agglutination. DSL prefers binding to (GlcNAc)2-4 and elutes with the sugar chitin hydrolysate. DSL binds well in the acidic pH range and its affinity decreases above pH 8.0. This agglutinin has blood group A, B, and O specificity. Studies have shown that DSA can act as a biomarker for neoplastic urotherial cells. Source: Jimson weed Blood Specificity: A, B, O Sugar Specificity: (N-Acetylglucosamine)2-4 Inhibitory Carbohydrate: Chitin Hydrolysate Divalent Ions: None Required Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile buffer or ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | ||
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