Griffonia (Bandeiraea) simplicifolia GS I-B4
Catalog No : USB-G8967-60
717.26€
0.00€
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| Product name | Griffonia (Bandeiraea) simplicifolia GS I-B4 | ||
|---|---|---|---|
| Catalog No | USB-G8967-60 | ||
| Supplier’s Catalog No | G8967-60 | ||
| Supplier | US Biologicals | ||
| Source antigen | Griffonia simlicifolia | ||
| Reactivity | |||
| Cross reactivity | |||
| Applications | |||
| Molecular weight | |||
| Storage | -20°C | ||
|---|---|---|---|
| Other names | |||
| Grade | Purified | ||
| Purity | |||
| Form | Supplied as a lyophilized powder from PBS, pH 7.2, 0.5mM calcium chloride. Reconstitute with buffer of choice. Note: Calcium is required for binding. 0.5mM calcium is the maximum concentration in buffer that will not form a white precipitiate. | ||
| Reactivity life | 12 months | ||
| Note | For reserch purpose only | ||
| Purity | |||
| Description | The seeds of Griffonia simlicifolia contain four distinct lectins. Two of these lectins, referred to as GS-I and GS-II, have been well characterized with regard to binding specificity and structure. GS-II is specific for terminal, non-reducing alpha or beta linked N-acetyl-D-glucosamine. This lectin is not blood group specific, unlike GS-I. GS-II is also a tetramer. It is composed of identical subunits. Each subunit has a single carbohydrate binding site for GlcNAc. GS-II is the only lectin isolated that is specific for only terminal GIcNAc residue. The subterminal saccharide does play an important role in lectin binding. Biological Activity: 20-30ug/ml is required to agglutinate fresh type B blood cells. Lectin activity against all blood types increases after neuraminidase treatment of cells. Carbohydrate Specificity: a-Galactose Inhibitory Carbohydrate: Gal a(1,3) > Methyl-a-galactoside . Galactose > Methyl-b-galactoside > N-acetylgalactosamine. Note: Griffonia simlicifolia (GS-1) exists as a mixture of 5 isolectins. Each isolectin is a tetramer consisting of different amounts of subunits termed A and B. The number after the subunit designation indicates the quantity of each subunit associated with the tetramer form. The GS-1B4 isolectin has four B-subunits. It has a specificiety for type B ethrocytes. Storage and Stability: Lyophilized powder may be stored at -20°C. Reconstitute to nominal volume by adding sterile buffer of choice, aliquot and store at -20°C. May be stored at 4°C for short-term only. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | ||
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