Phaseolus vulgaris P (PHA-P, Red Kidney Bean Phytohemagglutinin)

Catalog No : USB-P3370-60
388.52€
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Product name Phaseolus vulgaris P (PHA-P, Red Kidney Bean Phytohemagglutinin)
Catalog No USB-P3370-60
Supplier’s Catalog No P3370-60
Supplier US Biologicals
Source antigen Phaseolus vulgaris-P, Red Kidney Bean
Reactivity
Cross reactivity
Applications
Molecular weight 128
Storage -20°C
Other names
Grade Highly Purified
Purity ≥95% by SDS PAGE. Chromatographically purified. May contain traces of salt.
Form Supplied as a white, lyophilized powder. Reconstitute with assay buffer containing 0.05% sodium azide.
Reactivity life 12 months
Note For reserch purpose only
Purity ≥95% by SDS PAGE. Chromatographically purified. May contain traces of salt.
Description Lectins are derived from the extracts of plants, animals, viruses and microorganisms. They are known to agglutinate red blood cells. These agglutinins can select cell types according to blood group activities utilizing sugar-binding mechanisms. Lectins form precipitates with glycoconjugates and are useful for identifying or separating oligosaccharides with identical sugar compositions such as galactose, mannose or glucose. PHA consists of two molecular species, an erythroagglutinin (PHA-E) which has low mitogenic activity and high erythroagglutinin activity, and leucoagglutinin (PHA-L) which has high mitogenic and leucoagglutinating activity, but very low erythroagglutinating activity. PHA-E is not blood group specific, but agglutination can be inhibited by certain oligosaccharides. PHA-P is the protein form of PHA prior to separation and purification of erythroagglutinin and leucoagglutinin. PHA-M is the mucoprotein form. Carbohydrate Specificity: Recognizes a complex branched chain oligosaccharide structure Inhibitory Carbohydrate: Not inhibited by simple sugars Activity: Agglutinates 1ml 2% rabbit erythrocyte suspension at <10ug/ml Solubility: Soluble in most liquids Quality Control: SDS-PAGE, Agglutination assay. Storage and Stability: Lyophilized powder is stable for 12 months after receipt at -20°C Reconstituted product must be stored in aliquots at -20°C.