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Categories: Peptides & Proteins / Proteins

Product name			Phaseolus vulgaris (PHA-E) (FITC)
Catalog No			USB-518626
Supplier’s Catalog No			518626
Supplier			US Biologicals
Source antigen			Kidney bean
Reactivity
Cross reactivity
Applications
Molecular weight

Storage			4°C
Other names
Grade			Affinity purified
Purity			Affinity purified
Form			Supplied as a liquid in 10mM HEPES, 150mM NaCl, 0.1mM Ions, 0.02% sodium azide. Labeled with Fluorescein isothiocyanate (FITC).
Reactivity life			1 year
Note			For reserch purpose only
Purity			Affinity purified
Description			Phaseolus vulgaris Lectin (PHA-E) is labeled with fluorescein isothiocyanate (FITC) and has an appropriate number of fluorochromes bound to provide the optimum staining characteristics for this lectin. FITC conjugates have been used in a variety of immunohistochemical and flow cytometry applications. Fluorescein conjugates display a high rate of photobleaching, signal is sensitive to pH changes, broad fluorescence emission spectrum, and fluorescence quenching on conjugation to biopolymers. Source: Kidney bean Blood Specificity: A (-SA) Sugar Specificity: Complex N-Glycans Inhibitory Carbohydrate: Bovine Thyroglobulin, Acetic Acid Fluorescence: Green Divalent Ions: Ca++, Mn++ Excitation: 495nm Emission: 515nm Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.