Pisum sativum (PSA/PSL)

Catalog No : USB-518676
424.15€
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Product name Pisum sativum (PSA/PSL)
Catalog No USB-518676
Supplier’s Catalog No 518676
Supplier US Biologicals
Source antigen Pea
Reactivity
Cross reactivity
Applications
Molecular weight 53
Storage -20°C
Other names
Grade Affinity purified
Purity Affinity purified
Form Supplied as a lyophilized powder.
Reactivity life 2 years
Note For reserch purpose only
Purity Affinity purified
Description Affinity purified Pisum sativum lectin or agglutinin (PSA) is a heterodimer that consists of four subunits. It has a carbohydrate specificity towards alpha-linked mannose-containing oligosaccharides. It’s hemagglutinating activity can be inhibited by glucose, mannose, and sucrose. It does not have a blood group specificity. The N-terminal sequence of the lectin has shown some degree of homology compared to lectins from other legume species. Pisum sativum lectin has also shown mitogenic effect toward murine splenocytes and could inhibit activity of HIV-1 reverse transcriptase. PSA is often used to differentiate virally transformed from normal cells. Calcium and manganese ions are required for activity Source: Pea Blood Specificity: Non-specific Sugar Specificity: alpha-Mannose, alpha-Glucose Inhibitory Carbohydrate: alpha-Methylmannoside + alpha-Methylglucoside Divalent Ions: Ca++, Mn++ Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile buffer or ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.