Galectin-3 Binding Protein, Recombinant, Mouse, aa19-577 (MAC-2BP)
Catalog No : USB-G1044-56
682.78€
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| Product name | Galectin-3 Binding Protein, Recombinant, Mouse, aa19-577 (MAC-2BP) | ||
|---|---|---|---|
| Catalog No | USB-G1044-56 | ||
| Supplier’s Catalog No | G1044-56 | ||
| Supplier | US Biologicals | ||
| Source antigen | Recombinant, NS0 cells | ||
| Reactivity | |||
| Cross reactivity | |||
| Applications | |||
| Molecular weight | 63.3 | ||
| Storage | -20°C | ||
|---|---|---|---|
| Other names | |||
| Grade | Purified | ||
| Purity | ≥90% by SDSPAGE under reducing conditions and visualized by silver stain. Endotoxin: ≤0.1EU/ug (LAL) | ||
| Form | Supplied as a lyophilized powder from 20mM MOPS, 100Mm sodium chloride, 10mM Imidazole, 5% trehalose. Reconstitute with 167ul of sterile PBS. | ||
| Reactivity life | 6 months | ||
| Note | For reserch purpose only | ||
| Purity | ≥90% by SDSPAGE under reducing conditions and visualized by silver stain. Endotoxin: ≤0.1EU/ug (LAL) | ||
| Description | Galectin-3 binding protein (Galectin-3BP), called CyCAP (cyclophilin C-associated protein) in the mouse, is a secreted glycoprotein of the scavenger receptor cysteine-rich (SRCR) super family. Mouse Galectin-3BP is generally considered the paralog of human Galectin-3BP, which is also called MAC-2 binding protein (MAC-2BP), or 90kD tumor associated antigen (TAA90K or 90K). However, mouse Galectin-3BP binds cyclophilin C but not Galectin-3, while the opposite is true of human Galectin-3BP. The 577 amino acid, 77-95kD mouse Galectin-3BP contains an 18aa signal sequence and four definitive domains. Domain 1 is a group A scavenger receptor domain, domain 2 is a BTB/POZ domain that mediates dimerization, and domain 3 is an IVR domain that is also found following the POZ domain in Drosophila kelch protein. Little is known about domain 4. Mature mouse Galectin-3BP shares 88%, 69%, 67% and 65% aa identity with rat, human, dog and cow Galectin-3BP, respectively. The Galectin-3BP dimer interface residues are generally conserved between mouse and human, so linear dimers that occur in humans are also likely in the mouse. Human Galectin-3BP also forms ring-shaped decamers and dodecamers. Mouse Galectin-3BP is widely expressed and modifies innate immune reactions. It is stimulated in macrophages by adhesion, TNF-a or IFN-g. Mice deficient in Galectin-3BP secrete increased amounts of IL-12, TNF-a and IFN-g and have lower survival in response to endotoxin. Galectin-3BP is induced in rat skin fibroblasts, keratinocytes, macrophages and lymphocytes during wound healing, and wounded Galectin-3BP-deficient mice are slow to heal. Rat Galectin-3BP is upregulated after cerebral ischemia. Ligands for human Galectin-3BP include some other galectins, collagens and integrins, but remain to be tested for mouse Galectin-3BP binding. NSO-expressed recombinant protein corresponding to Thr19-Val577 of mouse Galectin-3 Binding Protein, with C-terminal 6x His tag. Accession Number: NP_035280 SDS-PAGE: 80-90kD, reducing conditions Biological Activity: Measured by its binding ability in a functional ELISA. When rmMAC-2BP is coated at 1ug/ml (100ul/well), rhGalectin-3 binds with an apparent KD <5nM. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 6 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer | ||
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