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Categories: Peptides & Proteins / Proteins

Product name			Erythrina cristagalli (ECA) (AP)
Catalog No			USB-E3453-20C
Supplier’s Catalog No			E3453-20C
Supplier			US Biologicals
Source antigen
Reactivity
Cross reactivity
Applications
Molecular weight

Storage			4°C Do Not Freeze
Other names
Grade			Purified
Purity			Purified
Form			Supplied as a liquid in 0.02M Sodium Bicarbonate, pH 9.0-9.5, 20-50% glycerol. Labeled with Alkaline Phosphatase (AP).
Reactivity life			12 months
Note			For reserch purpose only
Purity			Purified
Description			Pure Erythrina cristagalli lectin (ECA) from coral tree, Alkaline Phosphatase conjugated. Lectins are derived from the extracts of plants, animals, viruses and microorganisms and are known to agglutinate red blood cells. These agglutinins can select cell types according to blood group activities utilizing sugar-binding mechanisms. Lectins form precipitates with glycoconjugates and are useful for identifying or separating oligosaccharides with identical sugar compositions such as galactose, mannose or glucose. Carbohydrate Specificity: Galactose (beta1,4) N-Acetylglucosamine. Inhibitory Carbohydrate: N-Acetyllactosamine > Lactose > N-Acetylgalactosamine > Galactose. Activity: 1-2ug/ml will agglutinate neuraminidase treated human erythrocytes. Chemical Used for Conjugation: Alkaline Phosphatase. Storage and Stability: May be stored at 4°C before opening. DO NOT FREEZE! Stable at 4°C as an undiluted liquid. Dilute only prior to immediate use. Stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.