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Categories: Peptides & Proteins / Proteins

Product name			Erythrina cristagalli (ECA) (Biotin)
Catalog No			USB-E3453-20D
Supplier’s Catalog No			E3453-20D
Supplier			US Biologicals
Source antigen
Reactivity
Cross reactivity
Applications
Molecular weight

Storage			-20°C
Other names
Grade			Purified
Purity			Purified
Form			Supplied as a lyophilized powder in 0.01M phosphate, 0.15M sodium chloride, pH 7.2-7.4. Reconstitute with sterile dH2O PBS to 0.1-1mg/ml. Labeled with Biotin.
Reactivity life			12 months
Note			For reserch purpose only
Purity			Purified
Description			Pure Erythrina cristagalli lectin (ECA) from coral tree. Biotin conjugated. Lectins are derived from the extracts of plants, animals, viruses and microorganisms and are known to agglutinate red blood cells. These agglutinins can select cell types according to blood group activities utilizing sugar-binding mechanisms. Lectins form precipitates with glycoconjugates and are useful for identifying or separating oligosaccharides with identical sugar compositions such as galactose, mannose or glucose. Carbohydrate Specificity: Gal beta(1,4)GlcNAc Inhibitory Carbohydrate: N-acetyllactosamine > lactose > N-acetylgalactosamine > galactose Activity: 1-2ug/ml will agglutinate neuraminidase treated human erythrocytes. Chemical Used for Conjugation: Biotinyl N-hydroxysuccinimide ester (BNOHSE). Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile buffer or dH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.