Matrix Metalloproteinase 7, Recombinant, Human (MMP-7, Matrilysin, PUMP, EC 3.4.24.23)
Catalog No : USB-209084
417.25€
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| Product name | Matrix Metalloproteinase 7, Recombinant, Human (MMP-7, Matrilysin, PUMP, EC 3.4.24.23) | ||
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| Catalog No | USB-209084 | ||
| Supplier’s Catalog No | 209084 | ||
| Supplier | US Biologicals | ||
| Source antigen | Recombinant, E. coli | ||
| Reactivity | |||
| Cross reactivity | |||
| Applications | |||
| Molecular weight | 19.13 | ||
| Storage | -20°C | ||
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| Other names | |||
| Grade | Highly Purified | ||
| Purity | ~95% (RP-HPLC, SDS-PAGE) | ||
| Form | Supplied as a liquid in 10mM HEPES, pH 7.4, 5mM calcium chloride, 150mM sodium chloride. | ||
| Reactivity life | 12 months | ||
| Note | For reserch purpose only | ||
| Purity | ~95% (RP-HPLC, SDS-PAGE) | ||
| Description | Matrix metalloproteinase-7 (MMP-7) also known as matrilysin and PUMP (EC 3.4.24.23) cleaves a number of substrates including collagen types IV and X, elastin, fibronectin, gelatin, laminin and proteoglycans. MMP-7 is closely related to the stromelysin family members but is encoded by a different gene. MMP-7 is the smallest of all the MMPs consisting of a pro-peptide domain and a catalytic domain. It lacks the hemopexin-like domain common to other members of the MMPs. MMP-7 is secreted as a 28kD proenzyme and can be activated in vitro by organomercurials and trypsin and in vivo by MMP-3 to a 18kD active MMP-7 enzyme. Once activated, MMP-7 can activate pro-MMP-1 and pro-MMP-9 but not pro-MMP- 2. MMP-7 is widely expressed having been reported in elevated levels in cycling endometrium as well as in colorectal cancers and adenomas, hepatocellular carcinomas, rectal carcinomas, and ~50% of gliomas. Source: Recombinant protein corresponding to human MMP-7, a single, non- glycosylated, polypeptide chain, expressed in E. coli. Molecular Weight: ~19.13kD Specific Activity: ~3100IU/mg Unit Definition: One unit is defined as the digestion of 1ug Azocoll/min at 37°C. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: ELISA: can be used to coat plates at 1ug/ml and detected with antibodies (1:10-50K for neat serum, 0.5-1ug/ml for affinity pure) Western Blot: 1:1K-5K (neat serum), 1-10ug/ml (affinity pure antibody using ECL technique) Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | ||
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