Matrix Metalloproteinase-13, Recombinant, Human (MMP-13, Collagenase-3)
Catalog No : USB-209086
531.05€
0.00€
Shipping cost plus VAT not included , delivery in 7-14 business days
| Product name | Matrix Metalloproteinase-13, Recombinant, Human (MMP-13, Collagenase-3) | ||
|---|---|---|---|
| Catalog No | USB-209086 | ||
| Supplier’s Catalog No | 209086 | ||
| Supplier | US Biologicals | ||
| Source antigen | Recombinant, E. coli | ||
| Reactivity | |||
| Cross reactivity | |||
| Applications | |||
| Molecular weight | 27 | ||
| Storage | -20°C | ||
|---|---|---|---|
| Other names | |||
| Grade | Purified | ||
| Purity | ~90% (SDS-PAGE) | ||
| Form | Supplied as a liquid in 0.05M Tris-HCl buffer, pH 7.5, 0.2M sodium chloride, 5mM calcium chloride, 20uM ZnSO4, 0.1% BSA. | ||
| Reactivity life | 12 months | ||
| Note | For reserch purpose only | ||
| Purity | ~90% (SDS-PAGE) | ||
| Description | Latent recombinant human pro-collagenase (MMP-13) also called collagenase-3 truncated from C-terminal. Matrix Metalloproteinase-13 (MMP-13) is an enzyme that is a member of the MMP extracellular protease family. Extracellular protease enzymes, by virtue of their broad substrate specificities, play a role in both normal and disease states of tissue proliferation. Among the targets of MMP-13 are collagen, gelatin, entactin, pro-TNF-a, and chemokine SDF-11-4. MMP-13 is found in its latent form as a 52-56kD glycosylated proenzyme. Upon cleavage the 22-46kD MMP-1 becomes active in extracellular matrix remodeling. Because of the prominent role that MMP-1 plays in cell migration and metastasis, it is an important target for inhibition screening. Source: Recombinant protein corresponding to human MMP-13, a single, non-glycosylated, polypeptide chain, expressed in E. coli. Molecular Weight: ~27kD Biological Activity: Determined by the cleavage of fluorogenic peptide, 100ng of enzyme activated with APMA will digest 80% fluorogenic peptide substrate (0.1ml of 20uM solution) at 35°C for 30 minutes, (10ug=100 assays). Applications: Suitable for use as a standard for assaying MMP-13 or for screening inhibitors. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | ||
Product Details
© 2020 Imugex All Rights Reserved