Matrix Metalloproteinase-3, Recombinant, Human (MMP-3, Stromelysin-1, Transin-1)
Catalog No : USB-209087
417.25€
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| Product name | Matrix Metalloproteinase-3, Recombinant, Human (MMP-3, Stromelysin-1, Transin-1) | ||
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| Catalog No | USB-209087 | ||
| Supplier’s Catalog No | 209087 | ||
| Supplier | US Biologicals | ||
| Source antigen | Recombinant, E. coli | ||
| Reactivity | |||
| Cross reactivity | |||
| Applications | |||
| Molecular weight | 22 | ||
| Storage | -20°C | ||
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| Other names | |||
| Grade | Highly Purified | ||
| Purity | ~98% (RP-HPLC, SDS-PAGE) | ||
| Form | Supplied as a lyophilized powder. No preservative added. Reconstitute with sterile ddH2O >100ug/ml. | ||
| Reactivity life | 12 months | ||
| Note | For reserch purpose only | ||
| Purity | ~98% (RP-HPLC, SDS-PAGE) | ||
| Description | MMP-3 enzyme is also known as Stromelysin-1 or as Transin-1 which hydrolyzes natural collagen at physiological pH and temperature. It dissolves the intervertebral nucleus pulposus and annulus fibrosus of Herniated Lumbar Intervertebral Disk. MMP-3 hydrolyzes components of the extracellular matrix like proteoglycan, laminin, fibronectin, gelatin and collagen types III, IV and IX. It also activates pro-MMP-9 and pro-MMP-8 and super activates plasmin activated MMP-1. MMP-3 is secreted as a latent proenzyme and is activated by a variety of proteinases, e.g. plasmin, trypsin, chymotrypsin, cathepsin G or human neutrophil elastase. MMP-3 was found to be capable of activating the precursor of IL1-beta. Source: Recombinant protein corresponding to a single, non-glycosylated, polypeptide chain containing 230aa from human MMP-3, expressed in E. coli. Molecular Weight: ~22kD Specific Activity: ~1250mU/mg protein (H. Nagase, et al; J. Biol. Chem. 269, 20952, 1994). Unit Definition: One unit is defined as the amount of enzyme that hydrolyzes 1umol of MCA-Arg-Pro-Lys-Pro- Val-Glu-Nva-Trp-Arg-Lys(DNP)-NH2/min. at 37°C, pH 7.0. Storage and Stability: Lyophilized powder may be stored at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | ||
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