MMP-1, Recombinant, Human (CLG, Interstitial Collagenase, Matrix Metalloproteinase 1)
Catalog No : USB-215032
629.90€
0.00€
Shipping cost plus VAT not included , delivery in 7-14 business days
| Product name | MMP-1, Recombinant, Human (CLG, Interstitial Collagenase, Matrix Metalloproteinase 1) | ||
|---|---|---|---|
| Catalog No | USB-215032 | ||
| Supplier’s Catalog No | 215032 | ||
| Supplier | US Biologicals | ||
| Source antigen | Recombinant, E. coli | ||
| Reactivity | |||
| Cross reactivity | |||
| Applications | |||
| Molecular weight | 42.7 | ||
| Storage | -20°C | ||
|---|---|---|---|
| Other names | |||
| Grade | Highly Purified | ||
| Purity | ~90% (SDS-PAGE, HPLC) | ||
| Form | Supplied as a lyophilized powder in calcium chloride, sodium phosphate. No preservative added. Reconstitute with 100ul sterile ddH2O. | ||
| Reactivity life | 12 months | ||
| Note | For reserch purpose only | ||
| Purity | ~90% (SDS-PAGE, HPLC) | ||
| Description | MMP-1 (matrix metalloproteinase 1), otherwise known as interstitial collagenase, is a member of a family of secreted zinc-dependent endoproteases, which play a role in the degradation of extracellular matrix (ECM) components, including both fibrillar and non-fibrillar collagens, laminin, basement membrane glycoprotein and fibronectin. MMP-1 specifically cleaves types I, II, III, VII and X collagens, and also interacts with and cleaves the secreted viral Tat protein during HIV infection, resulting in a decrease in neuronal Tat's mediated neurotoxicity. MMP-1 is overexpressed in invasive melanoma, colorectal and oesophageal cancers, and has been implicated in arthritis, atherosclerotic lesion formation, and in the repair processes of the heart after myocardial infarction. Source: Recombinant protein corresponding to human MMP-1 expressed in E. coli. Molecular Weight: ~42.7kD containing the entire catalytic N-terminal domain and the C-terminal domain. Activity: MMP-1 activity was measured by its ability to cleave a chromogenic peptide MMP-1 substrate at RT. At an MMP-1 concentration of ~2.5ug/ml, 50% cleavage was achieved at an incubation time of ~25 mins. Applications: Suitable for use in Functional Assays. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Endotoxin Level: <1EU/ug Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | ||
Product Details
© 2020 Imugex All Rights Reserved