MMP1, Recombinant, Human, aa18-469, His-Tag (Interstitial Collagenase Isoform 1, CLG, CLGN)

Catalog No : USB-369101
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Product name MMP1, Recombinant, Human, aa18-469, His-Tag (Interstitial Collagenase Isoform 1, CLG, CLGN)
Catalog No USB-369101
Supplier’s Catalog No 369101
Supplier US Biologicals
Source antigen Recombinant, human from insect cell line
Reactivity
Cross reactivity
Applications
Molecular weight 53.1
Storage -20°C
Other names
Grade Highly Purified
Purity ~95% (SDS-PAGE)
Form Supplied as a liquid in 20mM MES, pH 5.5, 10mM calcium chloride, 100mM sodium chloride, 0.05% Brij35, 30% glycerol.
Reactivity life 6 months
Note For reserch purpose only
Purity ~95% (SDS-PAGE)
Description MMP1, also known as interstitial collagenase isoform 1, contains 4 hemopexin-like domains and is a member of the matrix metalloproteinase (MMP) family. It is capable of degrading a wide range of extracellular molecules and a number of bioactive molecules. It plays a central role in cell proliferation, migration, differentiation, angiogenesis, apoptosis and host defences. Source: Recombinant protein corresponding to aa18-469 from human MMP1, fused to His-Tag at C-terminal, expressed in insect cell. Molecular Weight: ~53.1kD (460aa) 50-70kD (SDS-PAGE under reducing conditions) AA Sequence: HSFPATLETQ EQDVDLVQKY LEKYYNLKND GRQVEKRRNS GPVVEKLKQM QEFFGLKVTG KPDAETLKVM KQPRCGVPDV AQFVLTEGNP RWEQTHLTYR IENYTPDLPR ADVDHAIEKA FQLWSNVTPL TFTKVSEGQA DIMISFVRGD HRDNSPFDGP GGNLAHAFQP GPGIGGDAHF DEDERWTNNF REYNLHRVAA HELGHSLGLS HSTDIGALMY PSYTFSGDVQ LAQDDIDGIQ AIYGRSQNPV QPIGPQTPKA CDSKLTFDAI TTIRGEVMFF KDRFYMRTNP FYPEVELNFI SVFWPQLPNG LEAAYEFADR DEVRFFKGNK YWAVQGQNVL HGYPKDIYSS FGFPRTVKHI DAALSEENTG KTYFFVANKY WRYDEYKRSM DPGYPKMIAH DFPGIGHKVD AVFMKDGFFY FFHGTRQYKF DPKTKRILTL QKANSWFNCR KNLEHHHHHH Endotoxin: ~1EU/1ug (LAL) Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.