MHC Class 1 Chain-related Gene B, Recombinant, Human (MICB)
Catalog No : USB-M3886-76
377.02€
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| Product name | MHC Class 1 Chain-related Gene B, Recombinant, Human (MICB) | ||
|---|---|---|---|
| Catalog No | USB-M3886-76 | ||
| Supplier’s Catalog No | M3886-76 | ||
| Supplier | US Biologicals | ||
| Source antigen | Recombinant, E. coli | ||
| Reactivity | |||
| Cross reactivity | |||
| Applications | |||
| Molecular weight | 37 | ||
| Storage | -20°C | ||
|---|---|---|---|
| Other names | |||
| Grade | Highly Purified | ||
| Purity | ≥ 95% as determined by RP-HPLC, anion-exchange FPLC and/or reducing and non-reducing SDS-PAGE Silver Stained gel. | ||
| Form | Supplied as a lyophilized powder without additives. | ||
| Reactivity life | 6 months | ||
| Note | For reserch purpose only | ||
| Purity | ≥ 95% as determined by RP-HPLC, anion-exchange FPLC and/or reducing and non-reducing SDS-PAGE Silver Stained gel. | ||
| Description | MICB (MHC class I chain-related gene B) is a transmembrane glycoprotein that functions as a ligand for human NKG2D. A closely related protein, MICA, shares 85% amino acid identity with MICB. These 2 proteins are distantly related to the MHC class I proteins. MICA and MICB (MICA/B) possess three extracellular immunoglobulin-like domains, but have no capacity to bind peptide or interact with 2-microglobulin. The genes encoding MICA/B are found within the major histocompatibility complex on human chromosome 6. The MICB locus is polymorphic with more than 15 recognized human alleles. MICA/B are minimally expressed on normal cells, but are frequently expressed on epithelial tumors and can be induced by bacterial and viral infections. MICA/B are ligands for NKG2D, an activating receptor expressed on NK cells, NKT cells, T cells, and CD8+ T cells. Recognition of MICA/B by NKG2D results in the activation of cytolytic activity and/or cytokine production by these effector cells. MICA/B recognition is involved in tumor surveillance, viral infections, and autoimmune diseases. The release of soluble forms of MICA/B from tumors down-regulates NKG2D surface expression on effector cells resulting in the impairment of anti-tumor immune response. Description: rHuMICB produced in E.Coli is a single, non-glycosylated polypeptide chain containing 326 amino acids and having a molecular mass of 37kD. The sequence contains the extracellular domain of the mature human MICB (amino acid residues Ala23 – Tyr312) MICB is purified by proprietary chromatographic techniques. Dimers and Aggregates: Less than 1% as determined by silver-stained SDS-PAGE gel analysis. Biological Activity: Recombinant Human MICB is fully biologically active when compared to standards. Measured by its ability to bind MICB antibody in ELISA. Endotoxin: ≤ 0.1ng/ug (IEU/ug) of Recombinant Human MICB. Protein Content: Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280 nm. 2. Analysis by RP-HPLC, using a standard solution of MICB as a Reference Standard. Solubility: It is recommended to reconstitute the lyophilized MICB in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. Stability: Lyophilized Recombinant Human MICB although stable at room temperature for 3 weeks, should be stored desiccated below -180C. Upon reconstitution MICB should be stored at 40C between 2-7 days and for future use below -180C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles. | ||
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