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Categories: Peptides & Proteins / Proteins

Product name			GRPEL1, aa28-217, Recombinant, Human (grpE Protein Homolog 1, Mitochondrial, HMGE, Mt-GrpE#1, GREPEL1)
Catalog No			USB-G3057-67
Supplier’s Catalog No			G3057-67
Supplier			US Biologicals
Source antigen			Recombinant, E.coli
Reactivity
Cross reactivity
Applications
Molecular weight			23.6

Storage			-20°C
Other names
Grade			Affinity Purified
Purity			~90% (SDS-PAGE).
Form			Supplied as a liquid in 20mM Tris-HCl, pH 8.0, 0.2M sodium chloride, 1mM DTT, 10% glycerol.
Reactivity life			6 months
Note			For reserch purpose only
Purity			~90% (SDS-PAGE).
Description			GRPEL1 is an essential component of the PAM complex, a complex required for the translocation of transit peptide-containing proteins from the inner membrane into the mitochondrial matrix in an ATP-dependent manner. This protein seems to control the nucleotide-dependent binding of mitochondrial HSP70 to substrate proteins. Source: Recombinant corresponding to aa28-217 of human GRPEL1 protein, fused to His-tag at N-terminus, expressed in E.coli. AA Sequence: MGSSHHHHHH SSGLVPRGSH MCTATKQKNS GQNLEEDMGQ SEQKADPPAT EKTLLEEKVK LEEQLKETVE KYKRALADTE NLRQRSQKLV EEAKLYGIQA FCKDLLEVAD VLEKATQCVP KEEIKDDNPH LKNLYEGLVM TEVQIQKVFT KHGLLKLNPV GAKFDPYEHE ALFHTPVEGK EPGTVALVSK VGYKLHGRTL RPALVGVVKE A Molecular Weight: 23.6kD (211aa) confirmed by MALDI-TOF Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.