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Categories: Peptides & Proteins / Proteins

Product name			MRRF, Recombinant, Human, aa56-262, His-Tag (Ribosome-recycling Factor, Mitochondrial Isoform 1, MRFF, MTRRF, RRF)
Catalog No			USB-138699
Supplier’s Catalog No			138699
Supplier			US Biologicals
Source antigen			Recombinant, E. coli
Reactivity
Cross reactivity
Applications
Molecular weight			25.1

Storage			-20°C
Other names
Grade			Highly Purified
Purity			~95% (SDS-PAGE)
Form			Supplied as a liquid in 20mM tris-HCl buffer, pH 8.0, 0.2M sodium chloride, 2mM DTT, 30% glycerol.
Reactivity life			6 months
Note			For reserch purpose only
Purity			~95% (SDS-PAGE)
Description			MRRF binds to the large ribosomal subunit in the cleft that contains the peptidyl transferase center. This protein is responsible for the release of ribosome from messenger RNA at the termination of protein biosynthesis. Also, it may increase the efficiency of translation by recycling ribosome from one round of translation to another. Source: Recombinant protein corresponding to aa56-262 from human MRRF at N-terminal, fused to His-tag expressed in E.coli. Molecular Weight: ~25.1kD (228aa) AA Sequence: MGSSHHHHHH SSGLVPRGSH MATKKAKAKG KGQSQTRVNI NAALVEDIIN LEEVNEEMKS VIEALKDNFN KTLNIRTSPG SLDKIAVVTA DGKLALNQIS QISMKSPQLI LVNMASFPEC TAAAIKAIRE SGMNLNPEVE GTLIRVPIPQ VTREHREMLV KLAKQNTNKA KDSLRKVRTN SMNKLKKSKD TVSEDTIRLI EKQISQMADD TVAELDRHLA VKTKELLG Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.