CS, Recombinant, Human, aa28-466, His-Tag (Citrate Synthase, Mitochondrial Precursor)

Catalog No : USB-218069
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Product name CS, Recombinant, Human, aa28-466, His-Tag (Citrate Synthase, Mitochondrial Precursor)
Catalog No USB-218069
Supplier’s Catalog No 218069
Supplier US Biologicals
Source antigen Recombinant, E. coli
Reactivity
Cross reactivity
Applications
Molecular weight 51.4
Storage -20°C
Other names
Grade Purified
Purity ~90% (SDS-PAGE)
Form Supplied as a liquid in 20mM Tris-HCl, pH 8.0, 0.15M sodium chloride, 1mM DTT, 10% glycerol.
Reactivity life 6 months
Note For reserch purpose only
Purity ~90% (SDS-PAGE)
Description Citrate synthase (CS) is a Krebs tricarboxylic acid cycle enzyme that catalyzes the synthesis of citrate from oxaloacetate and acetyl coenzyme A. It is found in nearly all cells capable of oxidative metablism. This protein is nuclear encoded and transported into the mitochondrial matrix, where the mature form is found. Source: Recombinant protein corresponding to aa28-466 from human CS, fused to His-tag at N-terminus, expressed in E.coli. Molecular Weight: ~51.4kD (462aa) Applications: Suitable for use in SDS-PAGE. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.