MRPL2, Recombinant, Human, aa84-202, His-Tag (39S Ribosomal Protein L2, Mitochondrial)

Catalog No : USB-218360
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Product name MRPL2, Recombinant, Human, aa84-202, His-Tag (39S Ribosomal Protein L2, Mitochondrial)
Catalog No USB-218360
Supplier’s Catalog No 218360
Supplier US Biologicals
Source antigen Recombinant, E. coli
Reactivity
Cross reactivity
Applications
Molecular weight 15.5
Storage -20°C
Other names
Grade Purified
Purity ~90% (SDS-PAGE)
Form Supplied as a liquid in 20mM phosphate, pH 8.0, 1mM EDTA, 2mM DTT, 50% glycerol.
Reactivity life 6 months
Note For reserch purpose only
Purity ~90% (SDS-PAGE)
Description Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. MRPL2 is a 39S subunit protein that belongs to the EcoL2 ribosomal protein family. Source: Recombinant protein corresponding to aa84-202 from human MRPL2, fused to His-tag at N-terminus, expressed in E.coli. Molecular Weight: ~15.5kD (142aa), confirmed by MALDI-TOF Applications: Suitable for use in SDS-PAGE. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.