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Categories: Peptides & Proteins / Proteins

Product name			ATPIF1, Recombinant, Human, aa26-106, His-Tag (ATPase inhibitor mitochondrial)
Catalog No			USB-349708
Supplier’s Catalog No			349708
Supplier			US Biologicals
Source antigen			Recombinant, E. coli
Reactivity
Cross reactivity
Applications
Molecular weight			12.2

Storage			-20°C
Other names
Grade			Highly Purified
Purity			~95% (SDS-PAGE)
Form			Supplied as a liquid in 20mM Tris-HCl, pH 8.0, 0.2M sodium chloride, 2mM DTT, 50% glycerol.
Reactivity life			6 months
Note			For reserch purpose only
Purity			~95% (SDS-PAGE)
Description			ATPase inhibitory factor 1, also known as ATPIF1, binds to the C-terminal region of a beta subunit of the F1-ATPase at low pH values and, via interference of the beta and gamma subunit interaction, ATPIF1 regulates the activity of the F1 (the hydrophilic catalytic core), F0 (the membrane embedded protein channel) ATPase. The overexpression of ATPIF1 in several human carcinomas further supports its participation in oncogenesis and provides insight into the altered metabolism of cancer cells, which includes the reprogramming of energetic metabolism toward glycolysis. Source: Recombinant protein corresponding to aa26-106 of human ATPIF1, fused to His-tag at N-terminal expressed in E. coli. Molecular Weight: ~12.2kD AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMGSDQS ENVDRGAGSI REAGGAFGKR EQAEEERYFR AQSREQLAAL KKHHEEEIVH HKKEIERLQK EIERHKQKIK MLKHDD Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.