HMGCL, GST-Tag, Recombinant (Hydroxymethylglutaryl-CoA lyase, Mitochondrial)

Catalog No : USB-500777
482.77€
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Product name HMGCL, GST-Tag, Recombinant (Hydroxymethylglutaryl-CoA lyase, Mitochondrial)
Catalog No USB-500777
Supplier’s Catalog No 500777
Supplier US Biologicals
Source antigen E. coli, Recombinant
Reactivity
Cross reactivity
Applications
Molecular weight 35
Storage -20°C
Other names
Grade Lysate
Purity Lysate
Form Supplied as a lyophilized powder in Tris-HCl, pH 6.8, 0.2M DTT, 4% SDS, 0.2% bromophenol blue, 20% sucrose. Reconstitute with 200ul sterile dH2O.
Reactivity life 6 months
Note For reserch purpose only
Purity Lysate
Description The protein encoded by this gene belongs to the HMG-CoA lyase family. It is a mitochondrial enzyme that catalyzes the final step of leucine degradation and plays a key role in ketone body formation. Mutations in this gene are associated with HMG-CoA lyase deficiency. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. Source: Partial length protein corresponding to of HMGCL, fused to GST-Tag at N-terminal, expressed in E. coli. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Molecular Weight: ~35kD Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.