NEDD8-AMC Substrate, Fluorogenic (NEDD8-aminomethylcoumarin)
Catalog No : USB-N0580-03
509.21€
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| Product name | NEDD8-AMC Substrate, Fluorogenic (NEDD8-aminomethylcoumarin) | ||
|---|---|---|---|
| Catalog No | USB-N0580-03 | ||
| Supplier’s Catalog No | N0580-03 | ||
| Supplier | US Biologicals | ||
| Source antigen | |||
| Reactivity | |||
| Cross reactivity | |||
| Applications | |||
| Molecular weight | 8.735 | ||
| Storage | -70°C | ||
|---|---|---|---|
| Other names | |||
| Grade | Highly Purified | ||
| Purity | ~95% (HPLC) | ||
| Form | Supplied as a liquid in DMSO. | ||
| Reactivity life | 12 months | ||
| Note | For reserch purpose only | ||
| Description | Prepared by the C-terminal derivatisation of NEDD8 with 7-amino-4-methylcoumarin. NEDD8- AMC acts as a useful and sensitive fluorogenic substrate for deNEDDylating enzymes, including NEDP1 and UCH-L3. It is a useful reagent for the study of deNEDDylating activity where detection sensitivity or continuous monitoring of activity is essential. Sequence: C-terminal derivatisation of NEDD8 with 7-amino-4-methylcoumarin Emission: 460nm Excitement: 380nm Molecular Weight: ~8.735kD Substrate Concentration: 10nM-1uM Enzyme Concentration: 10pM-100 nM Applications: Suitable for use in deNEDDylating Enzyme Activity. Other applications not tested. Storage and Stability: Aliquot to avoid repeated freezing and thawing and store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | ||
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