MAG (Siglec 4a) Fc Chimera, Recombinant, Rat
Catalog No : USB-M1748-50D
717.26€
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| Product name | MAG (Siglec 4a) Fc Chimera, Recombinant, Rat | ||
|---|---|---|---|
| Catalog No | USB-M1748-50D | ||
| Supplier’s Catalog No | M1748-50D | ||
| Supplier | US Biologicals | ||
| Source antigen | Recombinant, Mouse myeloma cell line, NS0 derived | ||
| Reactivity | |||
| Cross reactivity | |||
| Applications | |||
| Molecular weight | 120 | ||
| Storage | -20°C | ||
|---|---|---|---|
| Other names | |||
| Grade | Highly Purified | ||
| Purity | >95% (SDS-PAGE under reducing conditions and visualized by silver stain) Endotoxin: <1EU/ug (LAL). | ||
| Form | Supplied as a lyophilized powder from a 0.2um filtered solution in PBS. Reconstitute with 1ml sterile PBS. | ||
| Reactivity life | 6 months | ||
| Note | For reserch purpose only | ||
| Purity | >95% (SDS-PAGE under reducing conditions and visualized by silver stain) Endotoxin: <1EU/ug (LAL). | ||
| Description | MAG (Myelin Associated Glycoprotein), a type I transmembrane glycoprotein containing five Iglike domains in its extracellular domain is an adhesion molecule belonging to the immunoglobin superfamily. Within this superfamily, MAG, CD22, CD33, Schwann cell myelin protein, and sialoadhesin which bind specifically to cell surface glycan containing sialic acid residues define the Itype sialyl lectin subgroup, also called the sialoadhesin family. Sialoadhesins mediate diverse biological processes through recognition of specific sialyted glycans on cell surface. MAG is expressed on myelinating oligodenrocytes and Schwann cells, and preferentially recognizes α2, 3linked sialic acid on Olinked glycans and gangliosides. MAG exists as two isoforms which differ in the sequence and length of the cytoplasmic tail. The large form (71 kDa) and small form (67 kDa) arise from alternative spliced mRNAs. Although MAG might encounter haematopoietic cells and lymphocytes under pathologic conditions, it would normally be expected to interact with neuronal cells. It has been shown that MAG promotes axonal growth from neonatal DRG neurons and embryonic spinal neurons, but is a potent inhibitor of axonal regrowth from adult DRG and postnatal cerebellar neurons. MAG plays an important role in the interaction between axons and myelin. A soluble form of MAG containing the extracellular domain is released from myelin in large quantities and identified in normal human tissues and in tissues from patients with neurological disorders. This soluble MAG might contribute to the lack of CNS neuron regeneration after injury. Chimer structure: Rat MAG- linker- Human IgG1. The structure is disulfide linked homodimer, with a monomer Molecular Weight of ~81kD. Activity: Measured by its ability to inhibit neurite outgrowth of dissociated E13 chick embryonic dorsal root ganglia (DRG) neurons. Able to significantly inhibit neurite outgrowth when immobilized as a 3ul droplet containing 400ng on a nitrocellulose-coated microplate. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile buffer or ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 3 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | ||
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